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The monoclonal mouse anti-GST on the sensor chip CM5 by amine coupling, according to tIhgeGm2a aκn(uNfaaccatularieTr'essiqnuset,ru7,c0t0io0nRsU, a)nwda2s0im0 mRUoboilfizthede GST-tagged C-CPE were fixed on the sensor chip via the immobilized anti-GST antibodies in a sample...
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For monitoring of host chromo- some content, 0.25 μM primers (ACAAAACGCATATCGACCAGC; GCGATCCTTAACTTTGGTACGC) and an annealing/extension temperature of 60 °C were employed to amplify an 86 bp fragment present on the E. coli chro- mosomal d-1-deoxyxylulose 5-phosphate ...
Antibody discovery is bottlenecked by the individual expression and evaluation of antigen-specific hits. Here, we address this bottleneck by developing a workflow combining cell-free DNA template generation, cell-free protein synthesis, and binding measu
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The Man2c1rev and NEOREV primers were substituted with the following: Man2c1rev1, 5′-ACCAGGGTACTGGCTCTTGAC-3′ and NEOFOR, 5′-CAGGATGATCTGGACGAAGAG-3′, whereas the primer Man2c1for was maintained. The PCR reaction was modified as follows: denaturation at 98 °C for 3min; 40 ...
ACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATC TTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTT TGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAA Nature Communications | (2023)14:3897 8 Article https://doi.org/10.1038/s41467-023-38965-w CGCGATCCCGCGAAATTAATACGACTCACTATAGGGAGACCACAACG GTTTCCCTC...
The present invention is a DNA expression vector comprising: a toxP; a mutant toxO that blocks Fe-mediated regulation of gene expression; and a DNA sequence encoding a protein, wher