Samples were incubated with Fra1 polyclonal antibody (Product # PA5-95521) using a 0.5 µg/mL dilution, followed by a goat anti-rabbit IgG-HRP at a dilution of 1:10,000, and developed with enhanced chemiluminescence (ECL). View more ...
Antibodies used for immunoprecipitation were rabbit anti-mouse Fra-1(N-17)X (Santa Cruz Biotechnology, Santa Cruz, CA, USA) or isotype IgG antibody (Newprobe Bio- technology) as a negative control. Primers for the amplification of a Fra-1-binding site-specific, proximal region (nucleotides ...
Cryosections (5 μm) were made using Leica Kryostat (Model CM3050S, Leica, Wetzlar, Germany). After blocking in normal goat serum, the cryosections were incubated with rabbit anti-mouse von Willebrand Factor (vWF) antibodies and rat anti-mouse CD31 antibodies or monoclonal Hu177 antibodies (...
兔子可溶性P选择素(sP-selectin)Elisa试剂盒现货,rabbit soluble P-selectin,sP-selectin ELISA试剂盒,96T/48TCAS:3087-16-9价格,羊毛绿价格,25g1-(2'-甲氧基联苯)-N-甲基甲基胺价格,250mg小鼠可溶性肌球蛋白重链1(sMHC-1)Elisa试剂盒说明书|价格,Mouse soluble myosin hey chain 1,sMHC-1 ELISA试剂盒,96T/...
(rabbit anti-PI3K polyclonal antibody, catalog no. 4292; Cell Signaling Technology, Inc., Danvers, MA, USA; dilution, 1:500; rabbit anti-Akt polyclonal antibody, catalog no. 9272, Cell Signaling Technology; dilution, 1:300; rabbit anti-MDM2 antibody, 1:200 and rabbit anti-p53 antibody, 1...
(1:500; cat. no. ab2324; Abcam), caspase 9 (1:1,000; cat. no. ab32539; Abcam) and GAPDH (1:2,500; cat. no. ab9485; Abcam) at 4˚C overnight. Membranes were that incubated with horseradish peroxidase-conjugated goat anti-rabbit IgG secondary antibody (1:2,000; cat. no. ...
Fluorescein isothiocyanate (FITC)-conjugated anti-mouse antibody and Alexa Fluor® 564-conjugated anti-rabbit antibody (Invitrogen) were used for immunodetection. Immunostained cells were analyzed by fluorescence microscopy (ZEISS, Oberkochen, Germany). 4.8. Invasion Assay The invasion assay was ...