The FPbase FRET calculator shows spectral overlap and calculates the Förster distance (R0) for any FP pair in the database (provided the spectra and necessary attributes have been entered). The user may change values for the refractive index of the medium (n) or the orientation factor (κ...
This chapter serves as a general reference for using and searching the database and a guide to some of the more commonly used tools including the spectra viewer, custom microscope pages, and FRET calculator. Important caveats when evaluating the data are also discussed....