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Night of the Fox: Directed by Charles Jarrott. With George Peppard, Deborah Raffin, Michael York, David Birney. An American officer on a landing craft carrying plans for the Allied invasion of Europe in Normandy takes part on landing maneuvers, his craft
The high-profile platform sets out to bring international visibility to the wealth of ground-breaking talent working in film and TV in Germany, and shaping the future of the industry.This year’s selected talent are documentary writer-director Sarah Noa Bozenhardt (“Among Us Women”), actor-...
PCR was performed in 96-well plates (Biometra, Jena, Germany). Primers and dNTPs were digested prior to sequencing by enzymatic treatment using Shrimp Alkaline Phosphatase (M9910, Promega, WI, USA) and Exonuclease I (M0293, New England Biolabs, Ipswich, Madison, MA, USA). About 1.55 µ...
The experiment was performed in triplicate for each time point. Human TGF-β1 was purchased as lyophilised samples from R&D systems (Minneapolis, MN, USA). Sterile 4 mM HCl (Merck, Darmstadt, Germany) containing 0.1% (v/v) bovine serum albumin (Pierce) was added to prepare a stock ...
The samples were visualised by confocal laser scanning microscopy (Leica, Wetzlar, Germany). 2.13. β-catenin/TCF transcription reporter assay (TOP/FOPflash assay) To determine the activation status of Wnt signalling,β-catenin/TCF transcriptional activity was assayed by transfecting TOPflash/FOPflash...
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Anne Marie Fox. Camera and Electrical Department: Mary Shelley's Frankenstein. Anne Marie Fox is a native of southern California, educated at private Catholic schools with the exception of the three years spent attending Helmholtz Gymnasium in Heidelberg
Total RNAs were isolated from parental MSCs, reprogrammed MSCs, or human H9 ESCs with RNeasy mini kits (Qiagen, Hilden, Germany). Genomic DNAs were removed by DNase I (Qiagen) incubation. 0.5 μg RNAs were then reverse transcribed into cDNA using iScript reverse transcription supermix (...