HyperMu™ <KAN-1> Insertion Kit 10 Rxns使用说明书 HyperMu™ <CHL-1> Insertion Kit 10 Rxns使用说明书 pCC2 Forward Sequencing Primer 1 nmole使用说明书 我们将以高度的责任感和出色的产品质量为客户提供高品质的产品和实验技术解决方案。丰富的经验、优良的品质、充足的库存、准确的交货时间、极具竞争...
Μ l mixture of forward and reverse primers (10 μ mol · L-1), 0.2 μg l Taq DNA polymerase (5 u · MU L-1), the first chain 2 μ l cDNA synthesis product 翻译结果4复制译文编辑译文朗读译文返回顶部 µL forward and reverse primer mixture (10 µmol · L - 1) and 0.2 µ...
Amplicons were generated using 50 ng of gDNA in 50 μl reaction volumes containing 0.5 μM of primers. Loci 2 to 8 where amplified using the Phusion Green High-Fidelity DNA Polymerase (Thermo Scientific, Waltham, MA, USA) with the following parameters: 98 °C for 30 s, followed by 30 ...
Primers: mKate (CMV:output) forward: GGTGTCTAAGGGCGAAGAGC mKate (CMV:output) reverse: GCTGGTAGCCAGGATGTCGA 18S forward: GTAACCCGTTGAACCCCATT 18S reverse: CCATCCAATCGGTAGTAGCG. Flow cytometry To prepare samples in 96-well plates for flow cytometry, the following procedure was followed: medi...
This article traces the pioneering contributions of phage Mu to our current knowledge of how movable elements move/transpose. Mu provided the first molecular evidence of insertion elements in E. coli, postulated by McClintock to control gene activity in
Gene-specific primers were designed for polymorphic coding sequences of the seven candidate genes for reverse screening. Potential mutable sequence variations were identified for each candidate gene and queried for potential SNV via public domains. Selected differential genotypes for grain Fe density, inc...
Amplification and sequence of exon 19 to 25 of POLA1 from both human and mouse cell lines was amplified and sequenced using primers specified in Table S3. Crystal Violet Experiment 10 cm plates for each MMR deficient cell lines (3 million cells per plate) were treated with botezomib, MLN492...
Neuronal PC12 cells were treated with the Cdk inhibiting drugs roscovitine and flavopiridol and the JNK inhibitors SP600125 and DJnkI1 at 10 mM each (g) in the presence and absence of NGF for 16 h. cDNA derived from the cultures was subjected to quantitative PCR using Trib3 primers. Rat...
CD73, an ecto-5′-nucleotidase (NT5E), serves as an immune checkpoint by generating adenosine (ADO), which suppresses immune activation through the A2A receptor. Elevated CD73 levels in tumor tissues correlate with poor clinical outcomes. However, the cr
(Zymo Research), as previously described.23 PCR conditions and Sequenom EpiTYPER technology The PCR reactions were performed in a total volume of 5 μl using 1 μl of bisulfite-treated DNA, EpiTaq PCR Buffer 1 × , 0.4 μM of each primer, 0.3 mM dNTP mixture, 2.5 mM of MgCl2, 0.005...