In serial dilution, the density of cells is reduced in each step so that it is easier to calculate the concentration of the cells in the original solution by calculating the total dilution over the entire series. Serial dilutions are commonly performed to avoid having to pipette very small vol...
Milk thistle standard solution—Dissolve an accurately weighed quantity ofUSP Powdered Milk Thistle Extract RSin methanol,sonicate for 20minutes,and dilute with methanol to obtain a solution having a known concentration of about 0.7mg of extract per mL.Transfer 1mLof this solution to a 5-mLvolumet...
and allow theTest solutionto elute for not less than six times the retention time of apigenin-7-glucoside.Record the chromatograms,and measure the responses for all the peaks observed in the chromatogram of theTest solution.Calculate the percentage of apigenin-7...
To minimise the risk of osteoporosis, it is therefore recommended to calculate the dose as precisely as possible until the minimum effective dose is obtained. So, on account of the risks associated with over-dosing or under-dosing, not only of sodium salt of levothyroxine, but of thyroid ...
in mg,ofEchinacea purpureaAerial Parts taken;andrUandrSare the peak responses for the relevant analyte obtained from theTest solutionandStandard solution 2,respectively.Calculate the percentage of total phenols in the portion ofEchinacea purpureaAerial Parts taken by adding the individual percentages ...
Procedure—Proceed as directed in the test forContent of silymarinunderMilk Thistle,except to separately calculate the amount,in mg,of each relevant component of silymarin as silybin (C25H22O10)in the portion of Capsules taken by the formula: ...
Procedure— Separately inject equal volumes (about 10µL)of theAssay preparation and theStandard preparation into the chromatograph,record the chromatograms,and measure the responses for the major peaks.Separately calculate the percentages,on the anhydrous basis,of D-maltitol and D-sorbitol in the ...
and calculate the net weight of the Capsule contents.Transfer an accurately weighed portion of the Capsule contents,equivalent to about 1mg of fluoride,to a 100-mLvolumetric flask.Proceed as directed forAssay preparationin theAssay for fluoride,Method 2underOil-and Water-Soluble Vitamins with ...
of Eleuthero taken to prepare theTest solution;rUis the peak response of the relevant analyte obtained from theTest solution;andrSis the eleutheroside Bor eleutheroside Epeak response obtained from theStandard solution.Calculate the percentage of eleutheroside Band eleutheroside Ein the portion of ...
,and dilute with methanol to volume.Filter through a membrane having a 0.45-µm or finer porosity. Procedure—Proceed as directed in the test forContent of silymarinunderMilk Thistle,except to separately calculate the amount,in mg,of each relevant component of silymarin as silybin (C25H22O10...