Filters were then mounted onto glass slides with DAPI ProLong gold antifade rea- gent after drying and viewed at 1000X magnification on a Zeiss Axio Imager microscope. The number of DAPI pos- itive microbes was counted in 3 to 6 randomly chosen fields per disc, by two blinded scorers. The...
Of the 12 cells we observed under the microscope in the milk + lipase group, only one showed a bleb (included here to show the appearance of a bleb before rupture). All blebs from the formula + lipase group had already ruptured, as shown by loss of bleb spherical shape. B, bleb; P...
anti-rabbit (Cat#GB21303; Servicebio, China) at room temperature for 50 min away from light. Glass slides were prepared by mounting coverslips, and the DNA was counterstained using staining reagent containing DAPI (Servicebio). Image were obtained on NIKON ECLIPSE C1 fluorescence microscope (Nikon...
portion of gastric digesta was mixed with 0.2% (wt/vol) fluorescent dye rhodamine B (excitation and emission wavelengths of 543 and 625 nm, respectively) to stain the protein, placed on a concave confocal microscope slide, covered with a coverslip, and examined with a 63× magnification lens....
All images were captured by fluorescence microscope (Nikon, Japan). Statistical analysis Data are shown as mean ± SEM. Statistical significance among groups were tested by one-way ANOVA and post hoc analysis with the Least Significant Difference (LSD) test or the Games-Howell test. P <...
Particle size—Transfer 1drop to a microscope slide,and spread evenly,diluting with water,if necessary,to decrease the density of the field.Examine the slide under a microscope,equipped with a calibrated ocular micrometer,using about 400×magnification.Scan the entire slide,and note the size of ...
and allow to dry.Prepare four additional slides in the same manner from four additional containers.Examine each slide under a microscope equipped with a calibrated ocular micrometer,using 450×magnification.Focus on the particles of 5fields of view on each slide,near the center of the test ...
After washing with 0.25% PBS, the sections were incubated with secondary antibodies for 1 h, and DAPI (Beyotime) was used to visualize the nuclei. Finally, the sections were visualized under an epifluorescence microscope (LSM510-meta, Zeiss, Aalen Germany). The protein co-localization was then...
nification were counted under a light microscope[14]. Xenograft model of breast cancer bone metastases and drug treatment A xenograft model of bone metastases was established as previously described[11]. Briefly, after anaesthetization with 75 mg/kg of pentobarbital sodium, the mice were injected ...
Images were captured by Olympus microscope at ×200 magnification and analyzed by Image-Pro Plus 6.0 software. Immunohistochemistry The paraffin-embedded tumor tissues were sectioned (5 μm), deparaffinized, blocked with 3% hydrogen peroxide and washed with PBS. For immunostaining, sections were ...