Original Magnification: 200X. weeks28. We examined PC numbers in the jejunum and ileum of our pn day 15 rat pups by immunostaining for lysozyme. At both small intestinal sites, MM and MFGM supplemented pups displayed similar numbers of lysozyme-positive cells/crypt, whereas significantly fewer ...
Frozen liver tissues (central part of the left lateral lobe) were placed in optimal cutting temperature compound, cut into 8 μm sections and stained with Oil-Red O solution. Images were taken under Olympus IX71 Inverted microscope (Tokyo, Japan) at 200 × magnification. Biochemical analy...
Eventually these blebs rupture, killing the cells, and allowing entry of the life/death indicator, propidium iodide. Of the 12 cells we observed under the microscope in the milk + lipase group, only one showed a bleb (included here to show the appearance of a bleb before rupture). All ...
2% uranium acetate saturated alcohol solution and 2.6% lead citrate solution were stained for 8 min, avoiding carbon dioxide. We observed mitochondria, autophagosome and Rough endoplasmic reticulum of colonic ICC under a HITACHI HT7800 transmission electron microscope. 2.9. Western blot Proteins from ...
Where μ is the refractive index of the material of the lens R1 and R2 are the radii of curvature of the two surfaces of the lens. Lens formula: 1/v – 1/u = 1/f Linear magnification: m = hi/ho = v/u Power of a lens: P = 1/f if f is in meters Units of P: Dioptre ...
Images were captured by Olympus microscope at ×200 magnification and analyzed by Image-Pro Plus 6.0 software. Immunohistochemistry The paraffin-embedded tumor tissues were sectioned (5 μm), deparaffinized, blocked with 3% hydrogen peroxide and washed with PBS. For immunostaining, sections were ...
sections were stained by hematoxylin for 5 min. After washing, the sections were treated with eosin solution for 2 min for coloration, followed by ethanol dehydration and neutral gum sealing. Finally, the specimens were further observed and photographed under a BX53 microscope (Olympus, Takachiho, ...
Lactose crystals in powders were imaged with an optical microscope (Nikon Eclipse Ci-L, Nikon Instruments, Tokyo, Japan). The powder was dissolved in cold water and a drop was added on the microscopy slide, covered with a cover slip and imaged with 40 × magnification. 2.4.2. Confocal ...
For each group, every section was examined to acquire typical photographs (at a magnification of 100×). We counted the total number of cells in each glomerulus. When achieving photos, we ensured that the background lighting of each picture was consistent and the tissue was occupied the ...
The present work adopted a systems pharmacology-based approach to provide new insights into the active compounds and therapeutic targets of Bufei Yishen formula (BYF) for the treatment of chronic obstructive pulmonary disease (COPD). In addition, we established a rat model of cigarette smoke- and...