Nucleic acid extracts from cells pulse-labeled with [ 3H]uridine contain RNA that is associated with DNA in Cs 2SO 4 equilibrium density gradients. The amount of RNA bound to DNA was greatly reduced by repeated
RNA, unlike DNA, is single-stranded and uses the nucleotide base uracil instead of thymine. One specific form of RNA, called mRNA for being the 'messenger' RNA, carries the information from the DNA out of the nucleus and into the cytoplasm of a cell. The DNA is like the goods stored ...
Cucumber mosaic virus (CMV) often accompanies a short RNA molecule called a satellite RNA (satRNA). When infected with CMV in the presence of Y-satellite RNA (Y-sat), tobacco leaves develop a green mosaic, then turn yellow. Y-sat has been identified in the fields in Japan. Here, we ...
During embryonic development, epithelial cell blocks called somites are periodically formed according to the segmentation clock, becoming the foundation for the segmental pattern of the vertebral column. The process of somitogenesis has recently been rec
Table S1. SILAC Mass Spectrometry Analysis of Proteins Precipitated by b-isox from RNase-Treated U2OS Cells, Related to Figure 1 Table S2. RNA-Seq of Precipitated mRNAs from Mouse Brain and U2OS Lysate, Related to Figure 2 Table S3. GO Analysis of Pellet- and Supernatant-Enriched mRNAs, Re...
et al. Circular RNA is expressed across the eukaryotic tree of life. PLoS ONE 9, e90859 (2014). Article Google Scholar Wongsurawat, T., Jenjaroenpun, P., Kwoh, C. K. & Kuznetsov, V. Quantitative model of R-loop forming structures reveals a novel level of RNA–DNA interactome ...
For in situ hybridization experiments, DIG-labeled (Roche) antisense RNA was transcribed from a linearized fragment using DIG-labeled dNTPs. Four rounds of in situ hybridization experiments were performed. In total, five male anal fins (plus two female anal fins) were studied from P. sp. '...
Guanine-rich nucleic acid sequences are capable of folding into an intramolecular four-stranded structure called a G-quadruplex. When found in gene promoter regions, G-quadruplexes can downregulate gene expression, possibly by blocking the transcriptiona
4e–h). Re-analysis of our previously generated RNA-seq, chromatin immunoprecipitation sequencing (ChIP-seq) data from stem cell-derived endoderm34 suggested that the expression of N-cadherin as well as E-cadherin is regulated by Foxa2 (Fig. 2h and Supplementary Table 1). Taken together, ...
Liquid-solid transition, also known as gelation, is a specific form of phase separation in which molecules cross-link to form a highly interconnected compartment with solid – like dynamical properties. Here, we utilize RNA hairpin coat-protein binding sites to form synthetic RNA based gel-like ...