The AUG-dependent initiation took place only in the presence of 40S subunits and not in that of 80S ribosome couples. The poly(U)-dependent initiation could take place on 80S ribosome couples as well as on 40S subunits. Heating of 60S subunits removed their inhibitory effect on AUG-dependent...
Both are essential nucleolar proteins in yeast that are required for 18 S rRNA processing at sites A0, A1, and A2 and for the formation of small ribosomal subunits (34., 35., 36., 37.). They copurified with other components of the 90 S particle in tandem affinity purification (TAP) ...
persistence and/or cytoxic activity of tumor infiltrating cytotoxic T lymphocytes. As used herein, the term “tumor infiltrating cytotoxic T lymphocyte” refers to the pool of cytotoxic T lymphocytes of the patient that have left the blood stream and have migrated into a tumor. Accordingly, the ...
All samples were pooled with equal fractions and dried in a vacuum centrifuge. Seed weight and protein content measurements The individual seeds of all harvested materials were im- mediately weighed for average fresh weight using an analytical balance (TP-214; Denver Instrument, USA), while ...
D: Western blot analysis of lysates from either 20 or 40 nmol/L transfection of pooled ECT2 siRNA or nonsilencing (NS) siRNA. Both concentrations achieve efficient knockdown of ECT2 with little residual expression; 20 nmol/L of pooled ECT2 siRNA was used in all further experiments. E: ...
eIF4E binds the cap structure at the 5′ end of mRNAs and, through interaction with eIF4G, recruits the 43S preinitiation complex (consisting of the small ribosomal subunit (40S), the initiator tRNA, GTP, and a group of initiation factors) to the mRNA (reviewed in Preiss and Hentze, ...