The primary and secondary antibodies against rabbit and rat made of goats were acquired from Santa Cruz, California in the United States. Every other material and solution utilized in the study was molecular grade and of analytical quality. Cell Culture and Maintenance The HaCaT cells were obtained...
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Linguistic forms used in social interaction reflect and reconstruct identities, relationships, situations, and cultures. Whereas sociolinguistics has highlighted the social indexing of linguistic forms (Agha 2007;California Style Collective 1993;Eckert 2016), the field of variational pragmatics provides a com...
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In Santa Clara, the railroad corporations made the argument that because they wereoperating under special congressional legislation, they should be regarded as an extension of the federal government and therefore California could not tax them.90Field rejected this view (citing Dartmouth College), but ...
This work was supported in part by the California Institute for Regenerative Medicine (GC1R-06673-B) for funding, and sequencing and data repository support from C. Barragan, J. Ecker, J. Stuart, C. Fischer, W. Sullivan, P. Nejad, B. Aevermann and R. Scheuermann. We ...
A previous study of TDP-43 hollow condensates determined that conventional antibody-based immunofluorescence cannot be used to detect the components in the hollow core, as fixation produces a barrier that prevents antibody penetration44. Given the low dynamics of R475G hollow condensates, we felt ...
The blots were incubated in 2% BSA/Tri-buffered saline tween-20 (TBST) with primary antibodies overnight at 4 °C: anti-HSP90 (Santa Cruz, #sc-13119, 1:5000), anti-SEL1L (home-made, 1:10,000)44, anti-HRD1 (Proteintech, #13473-1, 1:2000), anti-OS9 (Abcam, #ab109510, 1...
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