The Integrator complex processes 3′-ends of spliceosomal small nuclear RNAs (snRNAs). Furthermore, it regulates transcription of protein coding genes by terminating transcription after unstable pausing. The molecular basis for Integrator’s functions re
At first glance, this may seem to be a modest undertaking since it is well known that harmonic oscillators can be constructed from a system of linear differential equations. Specifically, harmonic oscillations result when the Jacobian matrix of the linear system has pure imaginary eigenvalues (e.g...
Then we washed the cells twice with wash buffer A, and treated them for 30 min at 37°C with mixture of Duolink Ligation buffer and Ligase prepared according to manufacturer’s instructions. Cells were washed twice with wash buffer A, and treated for 100 min at 37°C with mixture of ...
D543-D552 CrossrefView in ScopusGoogle Scholar Cited by (5) Recent advances in genome editing of bloodstream forms of Trypanosoma congolense using CRISPR-Cas9 ribonucleoproteins: Proof of concept 2023, Experimental Parasitology Citation Excerpt : The CRISPR-Cas9 technique developed in 2012 (Jinek et...
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Eng. 2023, 279, 112061. [Google Scholar] [CrossRef] Prigogine, I.; Nicolis, G. Self-Organization in Non-Equilibrium Systems; Wiley: New York, NY, USA, 1977. [Google Scholar] Al-Shamery, K.; Parisi, J. (Eds.) Self-Organized Morphology in Nanostructured Materials; Springer: Berlin, ...
Gowthami, D.; Sharma, R.K. Influence of Hydrophilic and Hydrophobic Modification of the Porous Matrix on the Thermal Performance of Form Stable Phase Change Materials: A Review. Renew. Sustain. Energy Rev. 2023, 185, 113642. [Google Scholar] [CrossRef] Sundararajan, S.; Samui, A.B.; Ku...
Cells were then processed for genomic DNA and RNA using the AllPrep DNA/RNA Micro kit accord- ing to manufacturer instructions (Qiagen, 80284). To prepare the pro- tein lysate from the mouse RPE tissue, the dissected mouse posterior eyecup was transferred to a microcentrifuge tube containing ...
9 Reverse transcription was performed using the QuantiTect Reverse Transcription Kit (Qiagen) instructions. Briefly, gDNA was wiped out from samples containing 1 μg RNA. gDNA-free RNA samples were reverse transcribed following Qiagen’s instructions. Resulting cDNA was diluted 5-fold in DEPEC ...
Second strands were synthesized and adapters were added according to manufacturer’s instructions. Libraries were enriched by PCR amplification (12 cycles). PCR products were purified and size selected (400 bp) before sequencing using paired-end 100 bp, Illumina Hiseq 4000 sequencer. Sequencing ...