C-F qRT-PCR for colonic mRNA quantitative analysis of tnf-α (C), mcp-1 (D), il-6 (E) and il-1β (F). G-I qRT-PCR for colonic mRNA quantitative analysis of zo-2 (G), occludin (H) and claudin-1(I). Western blot and quantitative analysis of colonic ZO-2, Occludin and ...
Nature Communications14, Article number:3265(2023)Cite this article 6522Accesses 2Altmetric Metrics Abstract Acetyl-CoA utilized by histone acetyltransferases (HAT) for chromatin modification is mainly generated by ATP-citrate lyase (ACL) from glucose sources. How ACL locally establishes acetyl-CoA produc...
qRT-PCR Total RNA was isolated from cultured cells using a High Pure RNA isolation kit (Roche), reverse transcribed to cDNA with ReverTra Ace qPCR RT master mix (Toyobo), and assessed by qPCR using THUNDERBIRD Probe qPCR mix (Toyobo), a Universal ProbeLibrary probe (Roche), and a LightCy...
RNA extraction and qRT-PCR Three biological replicates as well three technical replicates from the same biological specimen were included in each analysis. mRNA was isolated using the MagMAX-96 Total RNA Isolation Kit (Thermo Fisher, Cat#AM1830) or the PicoPure RNA Isolation Kit (Thermo Fisher, ...
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中国现役轰-6K的数量是231架。 这一数据来源于美国军事网站《1945》的报道,该报道指出,尽管轰-6K在航程、最高速度、 美媒宣称中国有231架轰6,都是清一色崭新装备,数量全面超越... 根据美国最新掌握的情报的显示,目前中国列装的轰-6系列轰炸机为231架,它们绝...
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