3.5 × 104 cells were resuspended in 1 ml of 5iLAF-I medium and seeded into one well of an AggreWell 400 24-well plate (Stemcell,34411) pretreated with anti-adherence rinsing solution (Stem Cell Technologies, 07010) following the instructions and cultured under 20% O2 and 5% CO2 at 37...
As determined by deep RNA sequencing and bioinformatics, circRNAs are widely expressed RNA transcripts found in different species1,2,3,4,5,6. CircRNAs are inherently resistant to exonuclease activity (resulting in higher stability than linear RNAs) and often show tissue- or developmental stage-specif...
3.5 × 104 cells were resuspended in 1 ml of 5iLAF-I medium and seeded into one well of an AggreWell 400 24-well plate (Stemcell,34411) pretreated with anti-adherence rinsing solution (Stem Cell Technologies, 07010) following the instructions and cultured under 20% O2 and 5% CO2 at 37...