A messenger molecule, m-RNA, is necessary to carry the coded instructions from the DNA to the sites for protein biosynthesis. Since it is the base sequence of the DNA which carries these instructions, the base sequence of the m-RNA must be closely related to that of the DNA. This is ...
Cells were transfected with 3–4 μg of plasmid DNA complexed with LipofectAMINE 2000 (Invitrogen) according to the manufacturer's instructions. 24–48 h after transfection, cell cultures were aspirated, and cell monolayers were lysed in 250 μl of lysis buffer (1% Triton X-100, 10% glycerol...