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Genomic DNA was extracted using a DNA extraction kit (Qiagen) according to the manufacturer’s instructions. Approximately 10 μg of genomic DNA were digested with a 5-fold excess of NsiI or AgeI/EcoRV overnight at 37 °C. The samples were then run overnight in a 0.8% agarose gel....
Quantitative qPCR was performed using the Rotor-Gene 6,000 cycler (Corbett Life Science), and the KAPA SYBR FAST Universal qPCR Kit (Kapa Biosystems), according to the manufacturer’s instructions. The single copy rpoB gene was used as reference and standard to determine chromosome numbers. The...
Transcribed RNAs were then purified by micro Bio-Spin 6 chromatography columns (Bio-rad) according to the manufacturer’s instructions. The sizes of RNAs were checked by gel electrophoresis on a denaturing 6% polyacrylamide gel. 10 pM (3000 CPM) of labeled RNA was incubated at 90°C for 5 ...
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Processes 2023, 11, 720. [Google Scholar] [CrossRef] Qureshi, M.S.; Swarnkar, P.; Gupta, S. A supervisory on-line tuned fuzzy logic based sliding mode control for robotics: An application to surgical robots. Robot. Auton. Syst. 2018, 109, 68–85. [Google Scholar] [CrossRef] Sa...