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Heart Development Heart stem cells Cell Types Embryogenesis Cell Type Diversity Main The human heart comprises complex cardiac structures that are crucial for its function1,2. Disruption of these structures can lead to congenital heart disease, the most common birth defect, and adult structural heart...
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Bound RNA was extracted and reverse-transcribed into cDNA using Superscript III reverse transcriptase (Invitrogen) according to the manufacturer’s instructions, together with the RT primer (Table S4). To control for any nucleotide biases in the input random library, the input RNA pool (0.5 pmol)...
Twenty-four hours after transfection, the HEK-293T cells were har- vested to separate the proteins into cytoplasmic and nuclear proteins by the Nuclear and Cytoplasmic Protein Extraction Kit (P0027, Beyo- time Institute of Biotechnology, Haimen, China) according to the manufacturer's instructions....
We thank the Electron Microscopy and Light Microscopy Facilities of the MRC-LMB for support in data collection, Jake Grimmett and Toby Darling for computing support, Julia Mahamid for instructions on cryo-FIB milling, and Christopher Russo for help in using the Scios microscope. We thank Ruben ...
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