IRS instructions for Form 5472 You can complete the form by hand, but it’s better to do it on the computer: Don’t edit the PDF in your web browser (it might not save properly). Instead, download the PDF and save it to your computer. Then open it with a PDF application, like Ad...
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Form 5472 has been used for some time by domestic corporations with at least 25% foreign shareholders to provide information required under IRS Code Sections 6038A and 6038C. As of Jan. 1, 2017, pursuant to Treasury Regulations Section 1.6038A-1, the IRS treats a foreign-...
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7. For the avoidance of doubt, we do not accept any special instructions written on this Reply Form. 為免存疑,任何在本回條上的額外書寫指示將不予處理. #Corporate communications are all documents issued by the Company to holders of its securities for their information or action and include (...
RNA was extracted from cell pellets using the RNeasy kit (Qiagen) according to the manufacturer’s instructions. Complementary DNA was generated using equal amounts of RNA from each sample and the iScript complementary DNA synthesis kit (Bio-Rad) according to the manufacturer’s instructions. Reac...
Detection of biotin-labeled peptides was performed using Vectastain ABC reagent (Vector Laboratories) according to man- ufacturer's instructions. The wells were washed four times with binding buffer, then 100 μl of avidin-labeled peroxidase was added to each well, and the plate was ...
Detection of biotin-labeled peptides was performed using Vectastain ABC reagent (Vector Laboratories) according to man- ufacturer's instructions. The wells were washed four times with binding buffer, then 100 μl of avidin-labeled peroxidase was added to each well, and the plate was ...
HPDE and SUIT-2 cells were transfected with siRNAs by using RNAi MAX (Sigma-Aldrich) according to the manufacturer's instructions. Knockdown of integrin β4 was performed by using SMARTpool reagents (L-008011-00-0005, Horizon) and ON-TARGETplus nontargeting siRNA (Horizon) was used as a neg...