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Bound RNA was extracted and reverse-transcribed into cDNA using Superscript III reverse transcriptase (Invitrogen) according to the manufacturer’s instructions, together with the RT primer (Table S4). To control for any nucleotide biases in the input random library, the input RNA pool (0.5 pmol)...
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Total RNA was extracted from 100 mg of ground leaves using the RNeasy Plant Kit (QIAGEN France S.A.S, Courtaboeuf, France) following the manufacturer’s instructions. The quantity of the RNA was determined using a Qbit 4 Fluorometer (Thermo Scientific, Wilmington, NC, USA) and the RNA ...
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