After washing beads were boiled for 2 minutes in SDS sample buffer to isolate immunocomplexes and analysed by immunoblotting.Abbreviations BIR: Baculovirus inhibitor of apoptosis repeat CPC: Chromosomal passenger complex DIC: Differential interference contrast DMA: Dimethylenastron FACS: Fluorescent ...
Interestingly, our observation that mitotic SCEs arise independently of RAD52 implicates that we are not looking at a direct end-product of break-induced replication (BIR)89,90 or MiDAS70,91, which are both dependent on RAD52. Interestingly, MiDAS involves nuclease activity, and this pathway cou...
After wash- ing beads were boiled for 2 minutes in SDS sample buffer to isolate immunocomplexes and analysed by immunoblotting. Abbreviations BIR: Baculovirus inhibitor of apoptosis repeat; CPC: Chromosomal passenger complex; DIC: Differential interference contrast; DMA: Dimethylenastron; FACS: ...
In Table 1, probabilities of extinction have been expressed as the rates of extinction per million species years. Thus, marine gastropods typically last 10 million years, so a sample of 1 million marine gastropod species would be expected to experience 0.1 extinctions per year....
ResMap version 1.95 (Kucukelbir et al., 2014) was used to assess slices through local resolution estimates, using the default finest and coarsest resolution limits (2× and 4× pixel size, respectively). Map volumes and masks were visualized in UCSF Chimera version 1.13.1 (Pettersen et al....
Am Psychol 2000; 55:665-673. 8. Folkman S, Lazarus RS. An analysis of coping in a middle-aged community sample. J Health Soc Behav 1980; 21:219-239. 9. Folkman S, Lazarus RS. Manual for the Ways of Coping Questionnaire. Palo Alto, CA: Consulting Psychologists Press, 1988. 10. ...
After washing beads were boiled for 2 minutes in SDS sample buffer to isolate immunocomplexes and analysed by immunoblotting.Abbreviations BIR: Baculovirus inhibitor of apoptosis repeat CPC: Chromosomal passenger complex DIC: Differential interference contrast DMA: Dimethylenastron FACS: Fluorescent ...
A sample of the crude extract, the two supernatants and the proteins recovered in the immunoprecipitates (IPs) were resolved by SDS–PAGE, and western blotted with anti-AIRK2 antibody. Total, 1/30th of the amount of lysate added to the immunoprecipitate, which was loaded in its entirety. ...
under a binocular and then examined for grain size, morphology, and surface texture. The PGM grain was mounted to epoxy block and polished with diamond paste for further analysis. The composition and morphology of the sample were investigated at the Analytical Center for Multi-elemental and ...
2.2.1. CryoEM sample preparation and data acquisition The stock solution of BChE was diluted in the same buffer to a concentration of 0.3 μg/ml 3.0 μl of the BChE solution were immediately applied to glow-discharged Lacey 300 mesh grids (PELCO NetMesh) that was then plasma-treate...