Measurement of the force–extension curve for the mechanical unfolding process of a single protein molecule is expected to provide a value for the force necessary to deform the molecule. Integration of the observed curves can then yield an accurate estimate of the intra-molecular cohesive energy of...
To analyse the mechanics of intercalated DNA at approximately constant coverage, we acquired force-extension curves within a time that is much shorter than the minimum DNA–intercalator equilibration time (that is, the time corresponding to 1/keqat the lowest tension). First, DNA was incubated in...
Comparison to force-extension curves.Carrivain, PascalBarbi, MariaVictor, JeanMarc
a, Typical force-extension (FE) curves between integrin-functionalized tip with surface modified with no cRGD conjugated S (nRGD-S), cRGD-S, and cRGD-S with absent hairpin structure (cRGD-Sh-), respectively. For multiple pulling experiments, the hairpin was allowed to refold after one pulling...
We demonstrate that the experimental force-extension curves for short and long chain polymers are described by a unique universal model, despite the differences in chemistry and rate-dependence of transition forces.doi:10.48550/arXiv.1205.2867Fabio Manca...
The stretching and unfolding measurements performed on the semi-flexible DNA chain reveal (1) the softening of the uncondensed DNA contour length and (2) a mechanical behavior strikingly different from those previously observed: the force-extension curves of BSA-induced DNA condensates lack the "...
Theoretical or Mathematical/ Monte Carlo methods polymers/ Monte Carlo simulation description macroscopic force-displacement curves statistical ensembles thermodynamic limit arbitrarily long single molecules statistical mechanics single polymer force-extension relations/ A3620K Electronic structure and spectra of macr...
Figure 11.11 shows interparticle forces as a function of interparticle separation at different electrolyte concentrations with KNO3 at 25 °C, where the symbols such as cubes, triangles, and circles are the experimental results by the optical method, and the solid curves are those calculated from ...
uncontrolled attachment strategy, the number of protein domains being unfolded varies in different unfolding traces, as has been well-documented25. The force-extension curves of (GB1–G6)4are characterized by saw-tooth-like unfolding patterns (Fig. 1d) in which lower-force unfolding peaks are ...
To calculate the force that the condensate exerts on the non-condensed DNA, we used the worm-like-chain model, which relates λ-phage DNA’s extension and force. Upon addition of FoxA1, the amount of non-condensed DNA reduces, and the extension changes as follows:\(E = \frac{L}{{L_...