Measurement of the force–extension curve for the mechanical unfolding process of a single protein molecule is expected to provide a value for the force necessary to deform the molecule. Integration of the observed curves can then yield an accurate estimate of the intra-molecular cohesive energy of...
Comparison to force-extension curves.Carrivain, PascalBarbi, MariaVictor, JeanMarc
To analyse the mechanics of intercalated DNA at approximately constant coverage, we acquired force-extension curves within a time that is much shorter than the minimum DNA–intercalator equilibration time (that is, the time corresponding to 1/keqat the lowest tension). First, DNA was incubated in...
Rupture forces and unfolding forces were measured from the sharp decrease of the force from the force-extension curves and force-time traces. To estimate the unfolding step size, the DNA extension estimated by the eWLC fit was subtracted from the total extension. The step size was estimated ...
We find that despite complicated secondary structures, force-extension curves are typically smooth in quasi-equilibrium. We identify and characterize two sequence/structure-dependent mechanisms that, in addition to the sequence-independent entropic elasticity of the exterior single-stranded regions, are ...
Different biological molecules have now been analyzed (Kellermayer et al., 1997) and the accuracy of these techniques has sufficiently improved so that the theoretical models used to analyze force-extension curves must be refined. In particular, Bustamante et al. (1994) have shown that the force...
FIG 2 Force-extension curves for a 100-monomer polymer with a slip link in d,3 with its loop threaded through additional ,from top to bottom(m)0( 1)2( 3) and 4, rings This FE curve now satis,es a scaling form (r)f/R,(fR/,(2dT)),where the scaling function has the limits ...
Using the maximum-entropy method, we calculate the end-to-end distance distribution of the force-stretched chain from the moments of the distribution, which can be obtained from the extension-force curves measured in single-molecule experiments. If one knows the force expansion of the extension th...
2a). At low pulling forces, the force-extension curves fit well to a worm-like chain model (WLC) of a polymer with a persistence length of 50 nm, characteristic of a single dsDNA molecule23. At these forces, the macrocycle resides over the thermodynamically preferred fum station. Once ...
a, Typical force-extension (FE) curves between integrin-functionalized tip with surface modified with no cRGD conjugated S (nRGD-S), cRGD-S, and cRGD-S with absent hairpin structure (cRGD-Sh-), respectively. For multiple pulling experiments, the hairpin was allowed to refold after one pulling...