RNA polymerase I is responsible for the transcription of DNA to produce mRNAs in eukaryotes. a. True b. False True or false? RNA polymerase II initiates transcription of genes at the nucleotide in the DNA template that corresponds to the 5' end that is capped by the en...
Which of the following is (are) not required for DNA replication to occur? a. DNA polymerase b. nucleotides c. primers d. All are required. DNA Replication: All DNA must be able to replicate so that it can be divided...
However, since current Cas12a-based detection methods are limited to detecting double-stranded DNA (dsDNA) or ssDNA, a reverse transcription step and/or additional DNA pre-amplification steps are required for RNA detection23,24,25. In addition, CRISPR-Cas12a is typically activated by dsDNA target...
The biological logic of AIRE’s target specificity remains largely unclear as, in contrast to many transcription factors, it does not bind to a particular DNA sequence motif. Here we implemented two orthogonal approaches to investigate AIRE’s cis-regulatory mechanisms: construction of a convolutional...
Signals from cellular receptors are transduced by MAPKs to a wide variety of effector proteins, including transcription factors, which regulate cell functions according to environmental conditions. In this review, we focus on three subfamilies of MAPKs, namely, c-Jun N-terminal kinases (JNKs), ...
This review provides a comprehensive atlas of QTLs, genes, and alleles conferring resistance to 28 important diseases in all major soybean production regions in the world. Abstract Breeding disease-resistant soybean [Glycine max (L.) Merr.] varieties is a common goal for soybean breeding programs ...
We have found that genomic DNA must be actively removed via DNase I digestion, acid phenol:chloroform extraction, or lithium chloride precipitation. To illustrate this, qRT-PCR was performed on HeLa RNA isolated by several different methods (Table 2). Sample Kit +RT Ct –RT ...
They certified that CAR-T cells lacking DNMT3A retained the ability to proliferate and generate anti-tumor responses during prolonged tumor exposure [112]. Similarly, Good et al. established an in vitro model of antigen-driven CAR-T cell exhaustion and found that the modulation of transcription ...
Molecular docking is a computational technique that predicts the binding affinity of ligands to receptor proteins. Although it has potential uses in nutraceutical research, it has developed into a formidable tool for drug development. Bioactive substance
The development of delta cells is not well understood33. Mature delta cells can be identified bySstexpression (Extended Data Fig.5), but immature cells are much more difficult to identify.Hhexis the only widely accepted transcription factor required to maintain delta cell differentiation, and speci...