We performed PCR under the following reaction conditions: 10 μL 2X GoTaq PCR mix, 200 nM Fet FP, 200 nM biotinylated Fet RP, 1.5 μL recovered library, and H2O up to a final reaction volume of 50 μl, using the following cycling conditions: 95 °C for 3 min, ...
The catalytic substitution of phosphines for halide on CpFe(CO)2X(X Cl, Br, I) has been shown to proceed through an electron transfer chain catalysis mechanism. The reaction may be initiated by CpFe(CO)2(PR3) generated by photochemical or thermal cleavage of [CpFe(CO)2]2 or by addition...
Guided by gut sensory cues, humans and animals prefer nutritive sugars over non-caloric sweeteners, but how the gut steers such preferences remains unknown. In the intestine, neuropod cells synapse with vagal neurons to convey sugar stimuli to the brain within seconds. Here, we found that cholecy...
For dsGSTσ microinjection and soaking trials, the following reaction mix was used: 10 µL of iTaq universal Sybr Green supermix (Bio-Rad), 1 µL of 10 µM primers, 5 µL of H2O, 4 µL of 1/200 dilution cDNA (≈5 ng/µL). For the time-course and ...
1. PCRs were conducted for the left- and right-border separately in order to increase the probability of detection for all possible insertions, regardless of any disassociation or disagreement between the two borders. In the first PCR reaction, a semi-random genome walking primer is used in ...
The time dependence of Xe+ ion density was measured during the decay period of plasmas produced in xenon for reduced gas pressures varying from 0.10 to 4.0 Torr and a gas temperature of 300 °K. The reaction rate constant for the termolecular ion-neutral association reaction Xe+ + 2Xe → ...
After first identifying that a large proportion of host mitochondrial DNA remained, the host depletion process was optimised by increasing saponin concentration to 3% and scaling the reaction to allow more sample volume. Compared to non-depleted controls, the 3% saponin-based depletion protocol reduced...
This paper deals with the following degenerate and singular equation x(q)u(t) - (x(p)u(x))(x) = integral(a)(0) u(m)(x, t)dx - ku(n)(x, t) with non-local so... Zhou,J,Mu,... - 《Glasgow Mathematical Journal》 被引量: 18发表: 2010年 Blow-up phenomena for a nonloc...
reaction, information about both on- and off-target activation and reaction energies is highly valuable. Here, we use an automated workflow, based on the autodE program, to compute over 5000 reaction profiles for [3 + 2] cycloadditions involving both synthetic dipolarophiles and a set of...
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