Sections were then incubated with rabbit anti-Sct antibody (Ab; 1 : 500 dilution; Chemicon, Millipore, Billerica, MA), rabbit anti-Sctr Ab (1 : 500 dilution; Invitrogen, Carlsbad, CA), or rabbit anti-Fos Ab (1 : 500 dilution; Santa Cruz Biotechnol- ogy, Santa Cruz, CA) overnight ...
IRS-1 and GSK3β in C2C12 and L6 myotubes [37,38]. Lovastatin treatment inhibited the phosphorylated IRβ and IRS-1 levels in the skeletal muscle of rats fed a high-fat diet (HFD) [48]. However, other studies did not find significant effect of pravastatin on IRβ protein...
Secretin (Sct) is released into the circulation postprandially from the duodenal S-cells. The major functions of Sct originated from the gastrointestinal system are to delay gastric emptying, stimulate fluid secretion from pancreas and liver, and hence optimize the digestion process. In recent years,...
Imaging was performed in air and ambient conditions, using a Multimode VIII scanning probe microscope in tapping mode (Bruker, Billerica, MA, USA). The length and height distributions of the fibrils were characterized by analyzing the acquired images using the open source software FiberApp (Usov ...
or the MAP kinase inhibitor, UO126, overnight prior to harvest and determination of protein concentration. 20 μg of protein in a volume of 10 μl total lysate and 10 μl media were used for a rat/mouse GIP ELISA (Cat. #EZRMGIP-55K, Millipore, Billerica, MA, USA) according...
(RAST) server for annotation [30]. For microarray analysis, the duplicated purified DNA samples were further concentrated using an Amicon Ultracel-30 membrane filter (30,000 molecular weight cutoff, 0.5 ml, Millipore Corp. Billerica, MA) to a final volume of approximately 10–25 µl. ...
2.2. In Vivo Hormone Detection Plasma concentrations of leptin and GIP (glucose-dependent insulinotropic polypeptide) were assessed using antibody-immobilized beads specific to each hormone in a Milliplex® Map Kit (Millipore Corporation, Billerica, MA, USA) according to the manufacturer’s instructions...
The elemental composition for the compound was confirmed according to accurate mass and isotope rate calculations designated mSigma (Bruker Daltonics, Billerica, MA, USA). The accurate mass measurement was within 5 mDa of the assigned elemental composition, and mSigma values of <20 provided ...
Thus, 2 g of sample was homogenized with 10 mL of ultra-purified water (Milli-Q® Integral Water Purification System, EMD Millipore, Billerica, MA, USA) for 10 min in a Turratec crusher/homogenizer (TE-102-Tecnal®, Piracicaba, SP, Brazil). The suspension was centrifuged (4000× g...
2.4.5. X-Ray Diffraction Analysis Wide angle X-ray diffraction (WAXD) was performed on the biopaper samples using a Bruker AXS D4 Endeavour diffractometer from Bruker Corporation (Billerica, MA, USA). The samples were scanned, at room temperature, in reflection mode using incident CuKα ...