Untargeted metabolomics was performed using the SIEVE software (Thermofisher scientific). Briefly, after chromatogram alignment and retrieval of all the detected frames (e.g. ions), differentially accumulated metabolites (DAMs) were detected by a statistical analysis (one-way ANOVA plus Tukey’s pair...
To confirm PCR amplification from a single template, chromatograms were manually examined for multiple peaks, indicative of the presence of amplicons resulting from PCR-generated recombination events, Taq polymerase errors or multiple variant templates. All 441 sequences were aligned using ClustalW and ...
- 1990 () Citation Context ...al purity of the products was verified by high-pressure liquid chromatography (HPLC) with on-line beta-radiation counting detection by calculating the areas of the peaks obtained in the chromatograms =-=(24)-=-. For the syntheses of unlabeled alkanoylcholines,...
The LLOQ of the present UPLC–MS/MS method was 0.1 ng/ml. Typical chromatogram of spiked aconitine and oxpentifylline in blank plasma samples is shown in Fig. 1B. Linearity, precision, accuracy, recovery in plasma The standard curves for aconitine in rat plasma were linear in the concentra...
2015-05-30上传 Simultaneous determination of 33 amino acids and dipeptides in spent cell culture media by gas chromatography-flame ionization detection following liquid and solid phase extraction 文档格式: .pdf 文档大小: 504.31K 文档页数: 7页 ...
* A summary of this paper was presented a t the VIIIth Pan-American Congress on Pharmacy and Biochemistry in Caracas, Venezuela, in December, 1969. @ SAC and the authors.974 [Analyst, Vol. 97 Develop the chromatogram in a tank saturated with a chloroform - acetone (80 + 20) solvent ...
After quick sample preparation using acetonitrile as a protein precipitating agent, the chromatograms of brexpiprazole, DM-3411 and aripiprazole (internal standard, IS) were successfully separated by Acquity BEH C18 (2.1 mm 脳 50 mm, 1.7 渭m) column. The concentrations of the analytes were ...
(A) Chromatogram of the sample run. (B) Box plot showing the retention times for each of the individual 60 samples. Co-eluting light (C) and heavy transition (D) in a control sample from a TSQ Vantage raw file analysed using Skyline software. Dot plots of comparisons between the SRM ...
(A) Chromatogram of the sample run. (B) Box plot showing the retention times for each of the individual 60 samples. Co-eluting light (C) and heavy transition (D) in a control sample from a TSQ Vantage raw file analysed using Skyline software. Dot plots of comparisons between the SRM ...
a simple LC-MS/MS method was developed and validated for simultaneous determination of quercetin and kaempferol in beagle dog plasma. The conjugated forms of both analytes were hydrolyzed by β-glucuronidase and sulfatase followed by liquid-liquid extraction using methyltert-butyl ether. In addition,...