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Intrabodies, antibodies expressed within cells, offer an interesting way to target intracellular molecules, making them potentially useful for biotechnology and medicine. However, it remains controversial whether full-size IgG intrabodies expressed in th
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Whole-cell extracts were prepared from cells grown under nonstress and following exposure to hydrogen peroxide (0.1, 0.4 mM) for 10 or 30 min. Proteins were precipitated with TCA and free thiols modified by reaction with mPEG-MAL. Blots were probed with antibodies specific for Bcy1 (αFLAG)...