FokI-dCas9质粒结构是一个大肠杆菌表达载体,Tac强启动子可以驱动GST促溶标签和目的基因融合表达,LacI阻碍蛋白和Laco操作子使本质粒在没有加入IPTG之前禁止表达,防止其影响细菌生长。表达后的融合蛋白可用凝血酶蛋白酶切割,再过一次GST柱子即可清除掉GST标签。 产品相册FokI-dCas9质粒结构TTTCCTGCGTTATCCCCTGATTCTGTGGATA...
FokI-dCas9质粒 货号:#52970-20ul 货号产品名称规格库存价格数量购买 #52970-20ul FokI-dCas9质粒 20ul 立即咨询 + - 产品介绍 相关产品 》产品信息 货号 名称 规格 #52970 FokI-dCas9质粒 20ul 》产品描述 基因编辑 》产品简介 Plasmid #52970 》产品图谱及序列 质粒来源addgene,由于数量众多,图...
FokI-dCas9sgRNAMultiplex genome engineeringAll-in-one vectorLipofectionElectroporationPuromycin selectionSurveyor nuclease assayOff-target analysisCRISPR-Cas9 enables highly convenient multiplex genome engineering in cultured cells, because it utilizes generic Cas9 nuclease and an easily customizable single-guide ...
In this study, we usedFokI-dCas9 with two guide RNAs to integrate a 7.1 kilobase pair transgene into exon 9 of theGGTA1gene in porcine fetal fibroblasts. The modified cells lacked expression of the 伪Gal xenoantigen, and secreted an anti-CD2 monoclonal antibody encoded by the transgene. ...
中文名称:FOKI-DCAS9 中文同义词:FOKI-DCAS9 英文名称:FokI-dCas9 英文同义词: CAS号: 分子式: 分子量:0 EINECS号: 信息错误报告 您的Email: 按国家浏览FokI-dCas9国外供应商中国供应商 FokI-dCas9推荐供应商 建议您优先选择企业会员,我们对企业会员产品有严格审核。
We previously established a multiplex CRISPR-Cas9 assembly system for constructing an all-in-one vector simultaneously expressing multiple sgRNAs and Cas9 nuclease or other Cas9 variants including FokI-dCas9, which supersedes the wild-type Cas9 with regard to high specificity. In this chapter, we ...
FokI-dCas9sgRNAMultiplex genome engineeringAll-in-one vectorLipofectionElectroporationPuromycin selectionSurveyor nuclease assayOff-target analysisCRISPR-Cas9 enables highly convenient multiplex genome engineering in cultured cells, because it utilizes generic Cas9 nuclease and an easily customizable single-guide ...
FokI‐dCas9 mediates high‐fidelity genome editing in pigsdoi:10.1111/xen.12551Nella FisicaroEvelyn J SalvarisGayle K PhilipMatthew J WakefieldMark B NottleWayne J HawthornePeter J CowanWiley
Toward generating new dystrophic mouse mutants by homologous recombination using dimeric RNA-guided FokI-dCas9 nucleasesDuchenne muscular dystrophy (DMD) is a severe X-linked muscular disease that causes premature death and for which no cure exists. We have shown previously that in vitro treatment of...
Schematic representation of different dCas9 and FokI fusion variant architectures.Mustapha AouidaAyman EidZahir AliThomas CradickCiaran LeeHarshavardhan DeshmukhAhmed AtefDina AbuSamraSamah Zeineb GadhoumJasmeen Merzaban