High performance liquid chromatography with 9-fluorenylmethyl chloroformate (FMOC) permits detection of nitrogen-containing solutes including ectoine (1,4,5,6-tetrahydro-2-methyl-5-pyrimidinecarbocylic acid) from halophilic eubacteria. To enable ectoine derivatization with FMOC reagent the heterocycle of...
Butikofer U, Fuchs D, BosseJO and Gmur W: Automated HPLC-amino acid determination of protein hydrolysates by precolumn derivatization with OPA and FMOC and comparison with classical ion exchange chromatography. Chromatographia 1991; 31: 441-447....
Thismethod couldn't determine amino acids in Qingkailing injection accurately.[Key words]OPA-FMOC; on-line derivatization; amino acids; determination关于氨基酸的测定有柱前衍生化[1- 3]和柱后衍[4- 6],两种方法操作繁琐费时, 基于此方面生化法等的不足,本文建立了在线衍生化法测定氨基酸的含量,并将其...
A new method for the determination of amino acids is presented. It combines established methods for the derivatization of primary and secondary amino groups with 9-fluorenylmethoxycarbonyl chloride (F... 详情 Development and validation of a high-performance liquid chromatography-fluorescence detection met...
科研试剂;有机化工原料;化工原料;医药原料;偶联剂及保护剂;科研材料中间体;化工材料;通用生化试剂-氨基酸;化学试剂;Fluorenes & Fluorenones;Peptide Synthesis;Protection & Derivatization Reagents (for Synthesis);Protective Reagents (Peptide Synthesis);Synthetic Organic Chemistry;Fmoc-Amino acid series;peptides;...
Key words: amino acid;9-fluorenylmethyl chloroformate;high performance liquid chromatography;precolumn derivatization 氨基酸是生物大分子蛋白质的基本组成单位,是生命活动最基本的物质。在新陈代谢、神经传递等特殊的生理功能中发挥着重要作用 [1] 。氨基酸也是生物化学、生化制药、生物工程中的重要研究对象,因此涉及...
Keywords:FMOC-OSU,Aminoacids;RP-HPLC; FMOC-OSU AsPre-column Derivatising Pre-columnderivatization Res平ntForSepara咖.AndAna妙山ofAmino AcidsInHPLCDINGYa-yun.XIEMengzia (Analytical&TestingCenterofBeijingNomull 下载地址 文档纠错 收藏文档 下载帮助 下载源文档(pdf格式,0.09M) 需求调查 分享赚赏金 非法...
3.Study on Preparation of Fmoc Series Protected Amino Acids;Fmoc系列保护氨基酸的制备研究 4.Study on the Determination of Taurine in Foods and Health Foods by High Performance Liquid Chromatography after Derivatization with 9-fluolenylmeghyl Chloroformate;食品及保健食品中牛磺酸的FMOC柱前衍生—高效液相...
分析化学, 1998,26(10):1196— 1200. 5 FurstP,PollackI,GraserTA ,eta1.Appraisaloffourpre—columnderivatizationmethodsforthehigh—performance liquidchromatographicdeterminationoffreeaminoacidsinbiologicalmaterials[J].JChromatogr,1990,499:557— 569. 6 于泓,牟世芬.氨基酸分析方法 的研究进展 [J].分析化学,...
[关键词]OPA-FMOC; 在线衍生化; 氨基酸; 含量测定[中图分类号]R284. 1[文献标识码]A[文章编号]1005- 9903( 2012) 15- 0135- 04Determination of Amino Acids with OPA-FMOC by On-line DerivatizationSU Jian-kun, WANG Xue, LU Jian-qiu, QIAO Yan-jiang, GAO Xiao-yan*( Center of Scientific ...