刘必胜等:构建多顺反子表达载体的有效工具——FMDv2A767 lC—B FMD—AI2 FMD-OIK ERAv ERBV P个V一1 InsectViruses; CrPV DCV ABPV 工FV TaV Trypansomaspp.RepeatedSequences T.bruceiTSRI T.cruziAPEndOnuclease eCRotaviruses; PorcineRotavirus HumanRotavirus 2A—Mediated ...
The results showed that goat β-casein regulatory sequence can trigger hIL-2 and EGFP expression in mammary epithelial cells, and FMDV 2A facilitate expresson of hIL-2 and EGFP independently.%构建FMDV 2A介导的人白细胞介素2基因(hIL-2)和增强型绿色荧光蛋白(EGFP)基因双顺反子乳腺特异性表达载体,...
FMDV2A介导的乳腺上皮细胞双基因表达载体构建及表达 The aim of this study was to construct mammary gland specific bicistronic expression vector based on FMDV 2A, and verify the expression of sequences of EGF... 陈秋菊,王韵斐,崔易虹,... - 《畜牧兽医学报》 被引量: 0发表: 2011年 ...
The results showed that goat β-casein regulatory sequence can trigger hIL-2 and EGFP expression in mammary epithelial cells, and FMDV 2A facilitate expresson of hIL-2 and EGFP independently.%构建FMDV 2A介导的人白细胞介素2基因(hIL-...
sequencelisting<110>深圳出入境检验检疫局动植物检验检疫技术中心<120>一种用于fmdv和sva鉴别的试剂、方法及应用<130>17i25316<160>22<170>patentinversion3.3<210>1<211>20<212>dna<213>人工序列<400>1caggatgatgattggcagat20<...
通过使用由dayhoff,atlasofproteinsequencesandstructure,m.o.dayhoff编,5附录3:353-358,nationalbiomedicalresearchfoundation,washington,d.c.,usa开发,并且由gribskov(1986),nucl.acidsres.14(6):6745-6763加以标准化的评分矩阵,这个算法可应用于氨基酸序列。用以确定序列同一性百分比的这个算法的一示例性执行程序由...
A sequence corresponding to the critical 30 bp region that forms a stem-loop structure in IRES was also selected for investigation. The regions selected for shRNA were designated as D1, D2, D3, and D4. The virus in the supernatant was titrated in BHK-21 cells to determine infectivity. ...
(A) Depiction of the insertion of the FLAG epitope within P1 region of the FMDV genome, specifically within the coding sequence for capsid protein VP1. As indicated, to minimize disruption to the normal structure of the G–H loop, the second and third amino acids of the FLAG epitope, YK...
In total, 74.5% of the sequences could be aligned with tophat2 (v2.0.14; options: -N 2 –read-edit-dist 2 –b2-sensitive –no-coverage-search) on the ovine transcriptome (reference Ensembl 90 -Ovis aries 3.1). When trying to assign these alignments to genes using featureCounts (...
sequencelisting <110>河南农业大学 <120>o型fmdvvp1蛋白-铁蛋白融合蛋白、蛋白笼纳米颗粒及其制备方法 <130>无 <160>22 <170>patentinversion3.5 <210>1 <211>138 <212>prt <213>lumbricusterrestris <400>1 hismetlystyrtyrlysprolysphephetyrilelyssergluleu ...