The results showed that goat β-casein regulatory sequence can trigger hIL-2 and EGFP expression in mammary epithelial cells, and FMDV 2A facilitate expresson of hIL-2 and EGFP independently.%构建FMDV 2A介导的人白细胞介素2基因(hIL-2)和增强型绿色荧光蛋白(EGFP)基因双顺反子乳腺特异性表达载体,...
【目的】构建表达PRRSV GP5和M蛋白、且二者通过口蹄疫病毒(FMDV)2A序列连接的重组腺病毒,以期实现2个蛋白的同时表达,发挥GP5蛋白的病毒中和优势和M蛋白的细胞免疫优势作用。【方法】利用RT-PCR、间接免疫荧光和Western blot等方法,对获得的重组腺病毒(rAd-GP5-2A-M)进行检测。【结果】检测结果...
FMDV2A介导的乳腺上皮细胞双基因表达载体构建及表达 The aim of this study was to construct mammary gland specific bicistronic expression vector based on FMDV 2A, and verify the expression of sequences of EGF... 陈秋菊,王韵斐,崔易虹,... - 《畜牧兽医学报》 被引量: 0发表: 2011年 ...
Multicistronic lentiviral vectors containing the FMDV 2A cleavage factor demonstrate robust expression of encoded genes at limiting MOI pThe small and efficient 2A sequence can be used alone or in combination with an IRES for the construction of multicistronic lentiviral vectors which can... D Chin...
传统的构建多顺反子的工具,如IRES等,由于存在结构大,上下游基因表达差异显着等缺陷,严重限制了其应用.FMDV2A,因其具有结构小,剪切效率高等优点为多基因治疗带来了新的希望.主要介绍了FMDV2A的特性,"剪切"活力及其在构建多顺反子载体中的应用.关键词FMDV2A,多顺反子,基因治疗,肿瘤中图分类号Q789文献标识码A文章...
通过使用由dayhoff,atlasofproteinsequencesandstructure,m.o.dayhoff编,5附录3:353-358,nationalbiomedicalresearchfoundation,washington,d.c.,usa开发,并且由gribskov(1986),nucl.acidsres.14(6):6745-6763加以标准化的评分矩阵,这个算法可应用于氨基酸序列。用以确定序列同一性百分比的这个算法的一示例性执行程序由...
sequencelisting<110>深圳出入境检验检疫局动植物检验检疫技术中心<120>一种用于fmdv和sva鉴别的试剂、方法及应用<130>17i25316<160>22<170>patentinversion3.3<210>1<211>20<212>dna<213>人工序列<400>1caggatgatgattggcagat20<...
(based on MVA) vaccine, and related products, methods and uses. Specifically, the invention relates to a MVA vector modified by genetic engineering, which contains at least one heterologous nucleotide sequence encoding the FMDV protein antigenic determinant. The invention also relates to products, ...
共表达FMDVP1-2A、3C基因重组PRV TK～-转移载体的构建及表达 10. Cloning and Sequence Analysis of FMDV Structural Gene and Expression of VP1, Development of Indirect ELISA; FMDV结构基因的克隆与序列分析和VP1的表达及间接ELISA方法的建立 11. Prokaryotic Expression and Purification of Diagnostic Antigen 3ABC...
G ans u 730046 ) A bstract 1 0biective 1 In order to construct a recombinant foot and menth disease viru s Lactobacillus expression vector.1 Method 1 V P1 gene was amplifi ed by R T P CR from F MD V and sequenced .A ccording to the codon bias of LactobaciUus.the sequence of ...