Upon completion of exposure time, cells were harvested, washed twice with cold 1 X PBS and re-suspended in 0.1 ml of 1 X binding buffer (supplied with FITC Annexin V Apoptosis detection Kit). Then cells were incubated with 5 μl of FITC-Annexin V and PI for 10 min at room temperature...
Cell culture and exposure to nanoparticles The human lung adenocarcinoma (A549) cells were used in this study as in vitro models. A549 cells were procured from the American Type Culture Collection (ATCC, Manassas, VA) and cultured in DMEM F-12 culture medium (Life Technologies Pvt. Ltd., Inv...
スムーズなラチェット、最高のフィット感のFLUX BINDING --- ◆商品説明 SNSなどで話題沸騰のメーカー在庫切れのCV。2022モデルが予約の段階で3月に在庫切れとゆう前代未聞の話題の新型モデルの2023モデル! ハイバックが強化されアップグレードされたNEWMODELで...
Cells were harvested by centrifugation, subjected to osmotic shock, and GFP binding protein purified from the periplasmic fraction with Ni-NTA resin (Qiagen, Hilden, Germany) and an imidazole elution buffer using the manufacturer's recommended procedure (The QIAexpressionist: Protocol 11. Preparation ...