aspray paint 喷漆 [translate] aDomestic fisheries agencies 国内渔场代办处 [translate] acollateral 抵押 [translate] aLast summer Mike found a swimming factory near his house 麦克在他的房子附近去年夏天发现了游泳工厂 [translate] ayou need send to my SZ friend 您需要送到我的SZ朋友 [translate] a...
Modern light microscopy, including super-resolution techniques, has brought about a demand for small labeling tags that bring the fluorophore closer to the target. This challenge can be addressed by labeling unnatural amino acids (UAAs) with bioorthogona
Near-infrared PAINT localization microscopy via chromophore replenishment of phytochrome-derived fluorescent tag Kai Lu Tetsuichi Wazawa Takeharu Nagai Communications Biology(2024) Biomimetic NIR-II fluorescent proteins created from chemogenic protein-seeking dyes for multicolor deep-tissue bioimaging ...
But applications of PAINT to live cells are limited by impermeability and chemical toxicity of the probes and primed conversion FPs convert the emission from green to red upon exposure to blue and red/infrared lasers, making simultaneous multicolor imaging near impossible54,55. In Fig. 5, we ...
This DiB-split system presents a proof-of-principle demonstration of the potential of the lipocalin scaffold to create a split system. It is immediately applicable as a protein-PAINT23label of a smaller size for super-resolution imaging in living cells. The decrease of the tag size provided thr...
158 RESOLFT N/A 113 nm Slow 3 No Hela [63] Membrane-binding of proteins in EGF signaling pathway PAINT-PALM Proteinprotein interaction PAINT mEos3.2 default N/A PALM 35 nm N/A fast Yes 5 HeLa; CHO [67] Binding of PCNA and Mcm4 proteins to genomic DNA PAINT-PALM Protein-DNA ...