Use of Fluorescent Gel and \\{ATP\\} Bioluminescence Findings to Improve Discharge Room Cleaningdoi:10.1016/j.ajic.2013.03.153NancyC.HansenBrendaKelleyKimberlyPickardKelseyHooverDawnSDOSAmerican Journal of Infection Control
Osawa S, Yabuuchi E, Narano Y, Nakata M, Kosono Y, Takashina K, Tanabe T (1963) Pigment Production byPseudomonas aeruginosaon Glutamic Acid Medium and Gel Filtration of the Culture Fluid Filtrate. Japan J Microbiol 7: 87 CAS Google Scholar Osawa S, Yabuuchi E, Narano Y, Nakata M, ...
Chromatographic purifications were performed using Merck 9385 silica gel (pore size 60 Å; 230–400 mesh). Dulbecco’s minimum essential medium (DMEM), penicillin/streptomycin, phosphate-buffered saline (PBS), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), dimethyl ...
PCR amplified inserts were added at the N-terminus of N protein in pcDNA3.1 + plasmid, and purified with the NucleoSpin Gel and PCR Clean-up kit (Macherey–Nagel). The plasmid N and inserts were digested and purified. Ligation between these digested products was performed using T4 DNA ...
1a). The IBD was expressed in Escherichia coli and purified by affinity chromatography and gel filtration (Supplementary Fig. 1b, c). Multiangle light scattering (MALS) analysis showed that IBD appears as homodimer in solution (Supplementary Fig. 1d). Furthermore, the crystal structure of IBD ...
We concluded that InlC was produced and secreted when under the control of the constitutive Phyper promoter. Whole protein samples of the culture supernatant were then analyzed on a Coomassie-stained SDS-PAGE gel (Fig. 2 c). A band appears around 35 kDa for strain ΔinlCPhyper-inlC-GFP11...
Currently, classical sensing methods (e.g., gel electrophoresis [4], enzyme-linked immunosorbent assay (ELISA) [5], and polymerase chain reaction (PCR) [6]) often involve expensive antibodies, cumbersome procedures, and insufficient sensitivity, limiting their broad applications. Therefore, the ...
The NTri-Multi-Apts were characterized via agarose gel electrophoresis in 1 × TAE buffer at 130 V for 30 min. The loading sample was prepared by mixing 5 μL of DNA samples with 1 μL of loading buffer. In addition, the shape of the NTri-Multi-Apt was observed via atomic force mic...
(+control). The reaction was stopped by heat inactivating the enzyme for 3 minutes at 100° C. Unincorporated rNTPs were separated from the labeled RNA by gel sizing chromatography on a Sephadex G-25 column, after which the probe concentration was estimated from its absorbance at 260 nm. ...
After verified by enzyme cutting and gel electrophoresis,pAdxsi-GFP-NELL1 was amplified in HEK293 cells and purif ied by CsCl2 gradient purification,titrated using 50% tissue culture infective dose (TCID50) assay. The rat BMSCs were cultured and identifi ed byflow cytometry and directional ...