These absorbance measurements for both sample and standard should be performed with an instrument spectral bandpass Δμ, similar to that used for the fluorescence measurement, and the measured absorbance should
The Agilent 1260 Infinity Fluorescence Detector offers a fluorescence scan that delivers all spectral information previously obtained with a standard fluorescence spectrophotometer, independent of the LC fluorescence detector. Figure 34 on page 87 shows the complete information for quinidine as obtained with...
The Beckman DU spectrophotometer met those requirements. The second advance introduced the use of membrane filters for microfiltration of particles from seawater. Prior to the use of membrane filters, one had to resort to fine mesh netting or centrifugation....
The major difference between this detector and a double-beam spectrophotometer is that this detector employs only one flow cell rather than a simultaneous sample and reference flow cell pair. The detector obtains a fluorescence spectrum by performing the following types of scan on the flow cell: ...
UV-Vis spectra and the optical transmittance are recorded in a quartz cell (light path 10 mm) on a Shimadzu UV-2700 spectrophotometer equipped with a temperature controller. The UV-Vis spectra of film are recorded with film holder, and the thickness of film was measured as 0.5 mm. ...
Fluorescence analysis is a fast and sensitive method, and has great potential application in trace detection of environmental toxins. However, many important environmental toxins are non-fluorescent substances, and it is still a challenge to construct a
All the fluorescence spectra were recorded at room temperature at an excitation wavelength of 365 nm (Xenon lamp) on a Hitachi F-4500 fluorescence spectrophotometer with slit widths of 5/10 (Excitation/Emission, Response time: 0.5 s). The stock solution of the compounds was prepared in DMSO (...
The light transmission spectrum of this sheet sample was measured in the wavelength range of 290 nm to 2500 nm using a commercial spectrophotometer. Whether the optical absorption peak appeared in the respective wavelength ranges of 400 nm to 550 nm and 650 nm to 750 nm in the light ...
When the fluorescence spectrophotometer is used for detecting the sample substances in liquid chromatography, it is still more important to use a detector which is able to detect low light intensities, because the sample stays within the area of the detector only for a short time. That means ...
is the source of the fluorescence or phosphorescence. This basic process has virtually become an essential analytical tool in many fields because of the superb sensitivity that it offers over other systems. For example, with spectrophotometers, detecting micromolar concentrations is a major task. ...