Analysis of population structure, PCA, phylogenetic tree and AMOVA Population structure was estimated using Bayesian model-based approach implemented through the STRUCTURE program version 2.2 [62]. The putative number of a subpopulation (k) in the range of k = 1 to 5 was estimated using a ...
To investigate the evolutionary relationships of the RVE family between Rosaceae species andA. thaliana, we constructed a maximum-likelihood phylogenetic tree using putative protein sequences. Based on the topology of the phylogenetic tree and the classification of RVEs inA. thaliana, all proteins from...
Thus, our identification of putative HGTs does not appear to be due to contamination of the mRNA pool. Additionally, of the 31 Rafflesia HGT transcripts verified from our next-generation sequencing of genomic DNA, 16 show the presence of an intron (Additional file 7: Table S2 and Additional ...
(D) phylogenetic tree of OsFTL12 paralogues in rice. 2.2. Subcellular Localization Identified That the OsFTL12 Protein Is Targeted to the Nucleus The subcellular localization of the transcription factor OsFTL12 was predicted to be the nucleus by web-tool Cell-PLoc [24]. Next, in order to ...
The resulting tree was then annotated using iTOL (https://itol.embl.de/, accessed on 3 January 2023). 2.4. Gene Cloning and Sequence Analysis Total RNA was extracted from the tepals of I. laevigata using a kit (Kangweishiji, Taizhou, China), and the integrity was checked by ...