Flow cytometry-based cell sorting has its pros and cons. The advantages are clear: Researchers can separate cells based on a large number of variables that go beyond surface markers, such as size and metabolic status. This technique also has a high throughput, with the ability to analyze—lite...
Flow Cytometry Flow cytometry is a commonly used laser-based technique that is employed to count, sort, and phenotype cells in a single-cell suspension. Bio-Techne offers a broad-spectrum of antibodies and additional reagents and tools to streamline your flow cytometry experiments....
Direct killing of diseased cells is a hallmark function of NK cells. This protocol describes a flow-based assay to measure in vivo activated murine NK cells’ ability to kill target cells ex vivo. Existing published protocols for assaying ex vivo NK cell killing utilized the radioactive chromium...
8Depreter, B.; Philippé, J.; Meul, M.; Denys, B.; Vandepoele, K.; De Moerloose, B.; Lammens, T. (2018). Cancer-Related mRNA Expression Analysis Using a Novel Flow Cytometry-Based Assay. Clinical Cytometry, 94(4), 549–704. DOI: 10.1002/cyto.b.21593 ...
Here, we describe a protocol for the EPAM-ia method, which is based on flow cytometry for simultaneous isolation and analysis of endothelial cells, pericytes, astrocytes and microglia. This method is based on differential processing of NVU cell types using enzymes, mechanical homogenization and ...
Using flow cytometry, the present study aimed to investigate the immunophenotypic characteristics of malignant plasma cells (PCs) in the bone marrow of patients with primary systemic light chain amyloidosis (AL) compared with the characteristics of patients with multiple myeloma (MM). Flow cytometric ...
A BrdU-based flow cytometry assay was utilized to assess the impact of short-term tasquinimod treatment on myeloid cell recruitment to tumors. Additionally,... A Deronic,S Tahvili,T Leanderson,... - 《Bmc Cancer》 被引量: 4发表: 2016年 ProGraME: A novel flow cytometry algorithm for the...
Flow-cytometry-based physiological characterisation and transcriptome analyses reveal a mechanism for reduced cell viability in yeast engineered for increased lipid... H Peng,L He,VS Haritos - 《Biotechnology for Biofuels》 被引量: 0发表: 2019年 Mutations in the Arabidopsis Homolog of LST8/G beta...
The flow cytometry-based biosensor is capable of detecting less than 10 pM CT within 30 min. The signal generation strategy coupled with flow cytometry also provides a convenient method for kinetic studies of multivalent interactions. The surface density and the ratio of donor/acceptor-labeled GM1 ...
A Quantitative Flow Cytometry-Based Method for Autophagy Detection Across the Cell Cycle Autophagy is deregulated in cancer cells and often activated as a cellular stress response to anticancer therapies. Flow cytometry-based assays enable dete... VO Kaminskyy - 《Methods in Molecular Biology》 被引...