The most promising of these for flow cytometry is SNARF-1, which has a pKa of 7.4. These probes have dual emission wavelengths, one that is maximal under acidic conditions and the other that is maximal under alkaline conditions. This provides the theoretical advantage of increased sensitivity....
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capabilities. With spectral flow cytometry, the emission spectrum of every fluorescence molecule is captured by a set of detectors across a defined wavelength range. Every molecule’s fluorescent spectrum can be recognized, recor...
A single antibody (anti-TRBC1; JOVI-1 antibody clone) against one of the two mutually exclusive T-cell receptor β-chain constant domains was identified as a potentially useful flow-cytometry (FCM) marker to assess Tαβ-cell clonality. We optimized the TRBC1-FCM approach for detecting clonal...
A major route of penetration into our body after substance exposure is via the skin. Our aim was to generate robust culture conditions for a next generation human skin-on-chip model containing neopapillae and to establish proof-of-concept testing with the sensitizer, cinnamaldehyde. Reconstructed ...
Flow Cytometry Protocols Book ©2004 2nd edition View latest edition Overview Editors: Teresa S. Hawley, Robert G. Hawley Includes supplementary material:sn.pub/extras Part of the book series:Methods in Molecular Biology(MIMB, volume 91)
Flow Cytometry: First Principles, 2nd Edition Most research in the life sciences involves a core set of molecular-based equipment and methods, for which there is no shortage of step-by-step protocols. ... AS Gerstein 被引量: 13发表: 2001年 Flow Cytometry: First Principles, 2nd ed. By Ali...
(242) bacterial colonies were obtained from the DNA preparation of these 4805 sorted protoplasts, a ‘recovery-rate’ of 5%. Of those, only 1 out of 50 tested by flow cytometry in pair-wise challenges with the bait showed a positive BiFC signal (i.e. only 2%). Therefore, the plasmid ...
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Flow cytometry of testicular tissue from autopsies showed differences in the proportion of substained spermatozoa (1 CA), spermatids (1 CB), diploid 2C nuclei and premeiotic spermatozoa in the 4 C class. The evaluation of the histograms was performed wit