Multiparametric flow cytometry is a powerful diagnostic tool that permits rapid assessment of cellular antigen expression to quickly provide immunophenotypic information suitable for disease classification. This chapter describes a general approach for the identification of abnormal lymphoid populations by flow ...
CD11cMonocytes,HCL,LGL, activated T cells,MZL CD13Myeloid cells, rarepre-B-ALL CD14Monocytes CD15Granulocytes, Hodgkin's lymphoma CD16Granulocytes, NK cells,LGL CD19B cells,pre B-ALL, subset ofAML(AML1/ETOwith t(8;21)) CD20B cells, rare plasma cell myelomas ...
You are here We have many pages on the wiki for different diseases in which flow cytometry plays a role in diagnosis. Each page gives a brief description of the disease as well as the key elements in its flow cytometric diagnosis and evaluation. Each case also has an analysis protocol attac...
Flow cytometry has an essential role in the diagnosis and classification of acute leukemias.24,35,39Together with cytomorphology and cytochemistry, immunophenotyping is crucial for the detection and lineage assignment of blast cells in suspected samples, including the definition of acute leukemias of amb...
TCR-Vβ-R analysis is a sensitive method for detection of T-cell clonality and is useful for diagnosis and MRD detection in multiple specimen types. 展开 关键词: T-cell clonality Flow cytometry Vβ repertoire DOI: 10.1309/AJCPV2D1DDSGJDBW 年份: 2011 ...
vide differentiated leukemia cells their LGL resis- tance, it is essential to directly ascertain the pheno- type of the bound and unbound target cells at time of lysis. The information presented here expands on Ž . earlier work Zamai et al., 1998 showing that flow cytometry can be used ...
T-LGL leukemiaObjectives Flow cytometry immunophenotyping is limited by poor resolution of T-cell clones. A newly described antibody was recently used to distinguish normal peripheral blood T cells from malignant T-cell clones. Here, we evaluate this antibody as a new diagnostic tool for detecting ...
Clonality assessment through reverse transcription (RT)-PCR analysis of Vβ-Cβ transcripts and flow cytometry with a Vβ antibody panel covering more than 65% of Vβ domains was validated using 28 SB-defined clonal T-cell receptor (TCR)αβ+ T-ALL samples and T-cell lines. Next, the ...
The Turin Metropolitan Transplant Centre (CIC 305) includes four flow-cytometry laboratories assessing quality control on hematopoietic stem cells (HSC) with different instruments and operators. Therefore, the CD34+ enumeration assay should be validated on a regular basis. We describe here the ...
FLOW cytometryLYMPHOCYTESIMMUNE reconstitution inflammatory syndromeT-large granular lymphocytes (T-LGL) characterized by dim CD5 staining, although not completely understood, have unique roles in the immune system. Expansion of peripheral blood (PB) clonal T-LGL populations is associated with various ...