flow cell wash kit provides a highly effective means of removing a library that has been loaded onto a flow cell. once the library has been removed, the flow cell can be re-used immediately, or stored for later usage. the washing procedure is a simple two-step process and the kit ...
After performing a flow cell wash or storing your flow cell, we recommend using running a 'Flow cell check' to check number of available nanopores. Load your flow cell into the device with Storage Buffer (S) and start a Flow cell check to detect the number of active pores. For more inf...
10X Wash Buffer: (Part No. CS202123) 1 bottle containing 13 mL2. 5X Assay Buffer: (Part No. CS202124) 1 bottle containing 55 mL3. DNPH Solution: (Part No. CS204896) 1 bottle containing 2.5 mL4. Derivatization Control Solution: (Part No. CS204897) 1 bottle containing 2.5 mL FCCH...
Step 4:Wash with the same Flow Cytometry Staining Buffer used in sample staining, then centrifuge, and decant. Resuspend in Flow Cytometry Staining Buffer. Beads are ready to set compensation settings. Reference Guidelines for the use of flow cytometry and cell...
Figure 4. Staining profile of the ArC Amine Reactive Compensation Bead Kit components with 3 LIVE/DEAD Fixable Dead Cell Stain kits. (A)LIVE/DEAD Fixable Violet dye stained beadswere analyzed with 405 nm excitation, emission was coll...
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2 viability staining in 1 mL of PBS, add 1 μL of viability dye (incubate), according to the manufacturer’s protocol (LIVE/DEAD Fixable Dead Cell Stain Viability Kit, Molecular Probes) incubate for 15 min in the dark at RT 3 staining buffer wash wash cells in 3 mL of staining buffer...
Cell Recovery Solution, pipetted several hundred times, trypsinized with Trypsin-EDTA for 4 min at 37 °C and washed in medium to neutralise the Trypsin. Cells were passed through a 40 μm cell strainer (Corning, 352340) to ensure single-cell suspension, and their numbers counted ...
Annexin Binding Buffer(5×),for flow Cytometry 50ml R21951-30 Autibody Dilution Buffer 30ml R21951-100 Autibody Dilution Buffer 100ml R21936-100 Antibody Penetration Buffer 100ml T1231-100 ABTS缓冲液(pH=5.0) 100ml T1231-500 ABTS缓冲液(pH=5.0) 500ml R21917-8×100 Buffer...