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HRM results were similar for template levels of 7 and 70 ng when these cultivars were analyzed separately and together (Figure 6A). Figure 6 HRM analysis of the SNP in exon 4 of PpTFL1 using varied level of genomic DNA templates. A. Two levels (7 and 70 ng) of genomic DNA templates...
RNA samples were treated with DNase I at 37 ˝C for 30 min to remove DNA. A fragmentation buffer was added to break the mRNA-enriched RNAs into short pieces. With these short fragments as templates, first strands of cDNAs were synthesized using random hexamer primers. The second-strand ...
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