Extraction and purification methods in downstream processing of plant-based recombinant proteins EwelinaŁojewska, ...TomaszSakowicz, inProtein Expression and Purification, 2016 2.4Flocculants Flocculationis a
( 2014 ) Flocculation increases the efficacy of depth filtration during the downstream processing of recombinant pharmaceutical proteins produced in tobacco . Plant Biotechnol. J. 12 , 240 – 252 .Buyel JF, Fischer R (2014a) Flocculation increases the efficacy of depth filtration during the ...
In all these applications, after the yeast has fulfilled its respective function, cells are separated from the broth in the downstream processing. The flocculation phenom- enon is exploited in such applications for an easy, convenient and economical way to separate the flocs or aggre- gated yeast...
Downstream performance analysis: Completeness of flocculation (kinetics) Processing time and efforts for solid removal Liquid phase purity (including measurement of residual flocculant) Filtration capacity and efficiency Filter membrane breakthrough of debris or by-products Liquids in Flocculation Flocculants,...
like Alum (Al2(SO4)3), ferric ions etc. Cationic polymers and alkali substances are also used for flocculation which fluctuates pH[69]. However, removal of chemical flocculants from harvested biomass may increase the cost to further downstream stages in case of costly and quality conscious ...
Sun and spray drying, lyophilization, and drum drying are some of the drying techniques which are utilized for the drying of biomass for further downstream processing (Hadiyanto et al., 2023). Algal harvesting is considered the significant cost driver in the production of green fuel as it is ...
The initial downstream processing of intracellular microbial enzymes is usually conducted in the sequence: cell harvesting→cell disruption→ clarification→ initial enrichment. The efficiency of the conventional recovery route — most importantly the space-time yield, but also the activity yield — can ...
Existing downstream processing techniques, however, are not mature enough to overcome either techno-economic barriersor sustainability concerns, among which, energy-intensive microalgae harvesting is one of the biggest challenges. Biological microalgae harvesting has been demonstrated as the most promising ...
Flocculation can be defined as the process in which where a solute particle in a solution forms aggregates called floc [8]. The flocculation of microalgae can occur in four mechanisms acting alone or in combination; (i) Phenomenon of charge neutralization in which charged ions, polymers or coll...
The present invention provides compositions and methods for producing flocculation moieties in photosynthetic organisms. The photosynthetic organisms are genetically modified to eff