4m, o). Anti-CD31 antibody labeling of tumor vasculature showed the smallest red area in the wf-UMP group, indicating the suppression of tumor blood vessel proliferation (Fig. 4m, p). Collectively, wf-UMP effec
All antibodies were diluted in Intercept Antibody Diluent (Li-Cor, 927-66003). Western blots were visualized using an Odyssey Image Station (Li-Cor) and the Odyssey Application Software (3.0, Li-Cor). Statistics and reproducibility All live-cell experiments were performed with three independent ...
The concentration of the final library was determined with a Qubit 3.0 fluorometer (Invitrogen). The purified linear DNA library was circularized to generate a single-stranded DNA (ssDNA) library using a VAHTS Circularization Kit for MGI (Vazyme Biotech). The ssDNA library was amplified using a D...
Fab was prepared from monoclonal anti-FLAG M2 lgG2 antibody using a Pierce Fab preparation kit according to the manufacturer's protocol. To form the CYP102A1–Fab complex, FLAG-tagged CYP102A1 (8 μm) was incubated overnight at 4 °C in PBS solution with a 2-fold excess of the prepar...
We previously developed an in vitro system to monitor T-cell dysfunction induced by continuous bispecific antibody (BsAb) exposure in a clinically relevant B-cell lymphoma model [22]. It is unknown if AdCAR T cells react to CONT stimulation in the same way as conventional CAR T cells. Here...
Then, the cells were harvested and probed with PE-Intergrin β3 antibody, which was employed to lable the Intergrin β3 positive CRC cells. The cells were washed with PBS three times and then collected for flow cytometry analysis. The percentage of positive cells was evaluated by Flowjo ...
antibody (detection antibody) entrapped on the active SERS nanoparticles, and (2) a template called the adsorption substrate (which does not have to be a metal surface) functionalized with a binding antibody (an adsorption antibody) to bind the antigen SERS-tag complex. Using the SERS tag, ...