Flavin-dependent halogenases (FDHs) catalyze halogenation of arene and enol(ate) substrates. Herein, we reveal that FDHs engineered to catalyze site-selective aromatic halogenation also catalyze non-native bromolactonization of olefins with high enantioselectivity and near-native catalytic proficiency. ...
Although flavin-dependent halogenases (FDHs) are attractive for C–H bond activation, their applications are limited due to low turnover and stability. We have previously shown that leakage of a halogenating intermediate, hypohalous acid (HOX), causes FDHs to be inefficient by lessening halogena...
7.03.5.3Cytochromes P-450Reductase75 7.03.6Oxygen Reactions76 7.03.6.1Reaction of Free Flavins with O277 7.03.6.2Oxidases78 7.03.6.3Hydroxylases79 7.03.6.4p-Hydroxybenzoate Hydroxylase79 7.03.6.5MICAL81 7.03.6.6Tryptophan-7-Halogenase82 7.03.6.7ActVA and ActVB8337 ...
flavin-dependent halogenases; enzyme engineering; anion promiscuity; reaction engineering; co-factor recycling; biocatalysis Graphical Abstract1. Introduction In nature, over 5000 halogenated products have been described since their first discovery over 100 years ago including potent antimicrobial agents such...
In the present study, the pHMM was used to screen the bacterial associates of the Botryococcus braunii consortia (PRJEB21978), leading to the identification of several putative, flavin-dependent halogenase genes. Two of these new halogenase genes were found in one gene cluster of the ...
and l-amino acid oxidases, sugar oxidases, phenolic compounds hydroxylases, baeyer-villiger monooxygenases, flavin-dependent halogenases, flavin-dependent dehalogenases, styrene monooxygenases, bacterial luciferases, cellobiose dehydrogenases, prenylated flavoenzymes, ene-reductases, flavoenzymes in ...
Development of Halogenase Enzymes for Use in Synthesis 2018, Chemical Reviews Oxidative Cyclization in Natural Product Biosynthesis 2017, Chemical Reviews The contributory role of gut microbiota in cardiovascular disease 2014, Journal of Clinical Investigation View all citing articles on ScopusView...
Flavin‐dependent halogenases selectively introduce halogen substituents into (hetero‐)aromatic substrates and require only molecular oxygen and halide salts for this regioselective oxidative CH‐functionalization. Genomic analysis of Xanthomonas campestris pv. campestris B100 identified three novel putative ...
CRYSTAL STRUCTUREFlavin-dependent halogenases regioselectively introduce halide substituents into electron-rich substrates under mild reaction conditions. For the enzyme Xcc4156 from Xanthomonas campestris , the structure of a complex with the cofactor flavin adenine dinucleotide (FAD) and a bromide ion ...
Flavin-dependent halogenases require the activity of a flavin reductase for the production of reduced flavin, required by the actual halogenase. A number of flavin-dependent tryptophan halogenases have been investigated in some detail, and the first three-dimensional structure of a member of this...