9. Flag-tag epitope tag sequences Flag-tagFlag-tag amino acid sequence:DYKDDDDK Flag-tag DNA sequence:GAC TAC AAG GAC GAC GAT GAC AAG 3x Flag-tag3x Flag-tag amino acid sequence:DYKDHD-G-DYKDHD-I-DYKDDDDK 3x Flag-tag amino acid sequence:GAC TAC AAG GAC CAC GAC GGT GAC TAC AAG GAC...
Through DNA recombination technology, a bait vector containing the FLAG tag was constructed. Using yeast two‑hybrid technology, nanobodies against the FLAG tag were screened from a camel‑derived nanobody yeast library. Five single candidate antibody DNA sequences were screened from the yeast ...
Through DNA recombination technology, a bait vector containing the FLAG tag was constructed. Using yeast twohybrid technology, nanobodies against the FLAG tag were screened from a camelderived nanobody yeast library. Five single candidate antibody DNA sequences were screened from the yeast library. ...
后续检测主要通过FLAG-tag这段肽链形成的免疫决定簇与其单克隆抗体的特异性结合来实现。检测手段有免疫荧光(immunofluorescence),免疫印记(WesternBlotting)等。 随着生物技术的发展,科研人员可以通过DNA重组技术,构建包含目的基因以及表位标记的融合蛋白,进而通过特异性标签抗体对其鉴定与纯化,以达到研究的需求。00分享举报您...
1、aa sequence of FLAG tag: dykddddk (你可以查商品化质粒带的FLag tag确认一下 立个flag是什么意思? 说下一句振奋的话,结果往往与期望相反。简单地说,一个人被他自己说的话打脸了。你做了一件事,或说了一句话,为下面要发生的 <阿里巴巴>_frid标签,型号齐全,品质保障,厂家直销 <阿里巴巴>frid标签,专业厂...
ID sequence. 描述 Full length Clone DNA of Human tumor necrosis factor receptor superfamily, member 12A with Flag tag. 质粒启动子 Enhanced CMV promoter 载体 pCMV2-FLAG 限制性酶切位点 KpnI + XhoI (5.4kb + 0.44kb) 标签序列 FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG 测序引物 T7( ...
FLIP/CFLAR cDNA ORF Clone, Human, C-DDK (Flag®) tag 基本信息 基因 种属 Human NCBI 参考序列号 NM_015687.2 参考序列ORF长度 3642 bp 序列描述 Identical with the Gene Bank Ref. ID sequence. 描述 Full length Clone DNA of Human filamin A interacting protein 1 with Flag tag. 质粒...
Flag是一个由十几个氨基酸组成的蛋白标签,由目的蛋白(target protein)和标签(tag)共同构成的蛋白嵌合体,常标记于重组蛋白质的氨基端以帮助分析靶蛋白的生物学功能,用于目的细胞暂时不易得到或目的细胞转染效率非常低基因靶点的筛选[6]。本实验采用FLAG融合蛋白和foxp3基因共同构建靶基因重组真核表达质粒,瞬时转染进293...
The identification of TBX5-related regulatory sequences in genes essential for heart development is hampered by the absence of antibodies which allow precipitation of TBX5:DNA complexes. Employing CRISPR/Cas9 technology, we have inserted a FLAG-tag sequence at the end of exon 9 of the TBX5 gen...
IL-35 蛋白|IL-35 protein|IL-35(Human, Flag & His Tag)(产品说明)蛋白名称:IL-35 蛋白,IL-35 protein蛋白表达:IL-35 protein is produced with HEK293 Cells.蛋白纯度:IL-35 protein purity > 85 % as determined by SDS-PAGE.标签:Flag & His Tag蛋白构建:A DNA sequence encoding the human IL27...