目前市面上常见的3×Fag-tag标签肽有三种形式: 第一种序列①:DYKDDDDKGDYKDDDDKIDYKDDDDKH Sequence:DYKDDDDKGDYKDDDDKIDYKDDDDKH Length: 27 Mass: 3308.3341 Isoelectric point (pI): 3.85 Net charge: -9 Hydrophobicity: +79.53 Kcal * mol -1 Extinction coefficient1: 4470 M-1 * cm-1 Extinction coefficie...
Through DNA recombination technology, a bait vector containing the FLAG tag was constructed. Using yeast two‑hybrid technology, nanobodies against the FLAG tag were screened from a camel‑derived nanobody yeast library. Five single candidate antibody DNA sequences were screened from the yeast ...
9. Flag-tag epitope tag sequences Flag-tagFlag-tag amino acid sequence:DYKDDDDK Flag-tag DNA sequence:GAC TAC AAG GAC GAC GAT GAC AAG 3x Flag-tag3x Flag-tag amino acid sequence:DYKDHD-G-DYKDHD-I-DYKDDDDK 3x Flag-tag amino acid sequence:GAC TAC AAG GAC CAC GAC GGT GAC TAC AAG GAC...
Through DNA recombination technology, a bait vector containing the FLAG tag was constructed. Using yeast twohybrid technology, nanobodies against the FLAG tag were screened from a camelderived nanobody yeast library. Five single candidate antibody DNA sequences were screened from the yeast library. ...
flag标签作用 可以通过基因工程技术手段将所要研究的目的基因和FLAG-tag基因序列连接起来,可以连接在目的蛋白的C端或N端,然后将整合后的基因转入细胞中或胚胎干细胞抑或受精卵中。后续检测主要通过FLAG-tag这段肽链形成的免疫决定簇与其单克隆抗体的特异性结合来实现。检
1、aa sequence of FLAG tag: dykddddk (你可以查商品化质粒带的FLag tag确认一下 立个flag是什么意思? 说下一句振奋的话,结果往往与期望相反。简单地说,一个人被他自己说的话打脸了。你做了一件事,或说了一句话,为下面要发生的 <阿里巴巴>_frid标签,型号齐全,品质保障,厂家直销 <阿里巴巴>frid标签,专业厂...
FLIP/CFLAR cDNA ORF Clone, Human, C-DDK (Flag®) tag 基本信息 基因 种属 Human NCBI 参考序列号 NM_015687.2 参考序列ORF长度 3642 bp 序列描述 Identical with the Gene Bank Ref. ID sequence. 描述 Full length Clone DNA of Human filamin A interacting protein 1 with Flag tag. 质粒...
描述 Full length Clone DNA of Human RNA binding protein S1, serine-rich domain with C terminal Flag tag. 质粒 启动子 Enhanced CMV promoter 载体 pCMV3-C-FLAG 标签序列 FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG 测序引物 T7( 5' TAATACGACTCACTATAGGG 3' ) BGH( 5' TAGAAGGCACAGTCGA...
Flag是一个由十几个氨基酸组成的蛋白标签,由目的蛋白(target protein)和标签(tag)共同构成的蛋白嵌合体,常标记于重组蛋白质的氨基端以帮助分析靶蛋白的生物学功能,用于目的细胞暂时不易得到或目的细胞转染效率非常低基因靶点的筛选[6]。本实验采用FLAG融合蛋白和foxp3基因共同构建靶基因重组真核表达质粒,瞬时转染进293...
FLAG-Kir2.3-pGEMHE重组质粒DNA构建成功,没有碱基突变.FLAG标记短肽没有影响Kir2.3通道功能,FLAG-Kir2.3成功表达于非洲爪蟾卵母细胞,双电极电压钳可以记录到电流.免疫细胞化学实验证实FLAG标记短肽表达.结论 成功构建重组质粒DNA,FLAG标记短肽已与Kir2.3通道蛋白成功融合并有效表达.%Objective Flag tag will be ...