9. Flag-tag epitope tag sequencesFlag-tagFlag-tag amino acid sequence:DYKDDDDKFlag-tag DNA sequence:GAC TAC AAG GAC GAC GAT GAC AAG3x Flag-tag3x Flag-tag amino acid sequence:DYKDHD-G-DYKDHD-I-DYKDDDDK3x Flag-tag amino acid sequence:GAC TAC AAG GAC CAC GAC GGT GAC TAC AAG GAC CAC ...
目前市面上常见的3×Fag-tag标签肽有三种形式: 第一种序列①:DYKDDDDKGDYKDDDDKIDYKDDDDKH Sequence:DYKDDDDKGDYKDDDDKIDYKDDDDKH Length: 27 Mass: 3308.3341 Isoelectric point (pI): 3.85 Net charge: -9 Hydrophobicity: +79.53 Kcal * mol -1 Extinction coefficient1: 4470 M-1 * cm-1 Extinction coefficie...
Through DNA recombination technology, a bait vector containing the FLAG tag was constructed. Using yeast two‑hybrid technology, nanobodies against the FLAG tag were screened from a camel‑derived nanobody yeast library. Five single candidate antibody DNA sequences were screened from the yeast ...
Through DNA recombination technology, a bait vector containing the FLAG tag was constructed. Using yeast twohybrid technology, nanobodies against the FLAG tag were screened from a camelderived nanobody yeast library. Five single candidate antibody DNA sequences were screened from the yeast library. ...
描述 Full length Clone DNA of Human collagen, type VIII, alpha 1 with N terminal Flag tag. 质粒 启动子 Enhanced CMV promoter 载体 pCMV3-SP-N-FLAG 标签序列 FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG 测序引物 T7( 5' TAATACGACTCACTATAGGG 3' ) BGH( 5' TAGAAGGCACAGTCGAGG 3' ...
FLIP/CFLAR cDNA ORF Clone, Human, C-DDK (Flag®) tag 基本信息 基因 种属 Human NCBI 参考序列号 NM_015687.2 参考序列ORF长度 3642 bp 序列描述 Identical with the Gene Bank Ref. ID sequence. 描述 Full length Clone DNA of Human filamin A interacting protein 1 with Flag tag. ...
Flag多肽(Flag-tag Peptide)是编码8个氨基酸的亲水性多肽(DYKDDDDK),含有肠激酶酶切位点,可用于免疫亲和层析。Flag多肽可在非变性条件下洗脱蛋白,有多种抗体可识别Flag多肽,如M1、M2和M5。 Cas No. (Cas No.):98849-88-8 多肽序列(Amino acid sequence):H-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-OH ...
flag标签作用 可以通过基因工程技术手段将所要研究的目的基因和FLAG-tag基因序列连接起来,可以连接在目的蛋白的C端或N端,然后将整合后的基因转入细胞中或胚胎干细胞抑或受精卵中。后续检测主要通过FLAG-tag这段肽链形成的免疫决定簇与其单克隆抗体的特异性结合来实现。检
that is made up of three tandem repeats of FLAG-like sequences (Hernan et al., 2000). Other commonly used epitope tags are the HA-tag and the c-Myc tag (Fritze and Anderson, 2000). 3.3.2 The S-tag S-tag, reviewed by Raines et al. (2000), takes advantage of the tight ...
protein complexes with multiple subunits.A Flag-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a Flag-tag to this protein allows one to follow the protein with an antibody against the Flag sequence....