Anti-FLAG M2 Magnetic Beads are 4% agarose beads bound with the exclusive Sigma Anti-FLAG M2 (mouse monoclonal) antibody.
beadsflagmagneticresinfusionsigma 系统标签: beadsflagmagneticresinfusionsigma ANTI-FLAG®M2MagneticBeadsCatalogNumberM8823StorageTemperature–20°CTECHNICALBULLETINProductDescriptionANTI-FLAGM2MagneticBeadsarecomposedofthemurinederived,ANTI-FLAGM2monoclonalantibodyattachedtosuperparamagneticironimpregnated,4%agarosebeadswit...
Anti-FLAG M2 Magnetic Beads(抗FLAG M2 磁珠),1ml分享给好友: 详细描述 目录号:M8823-1ML 品牌:Sigma-Aldrich 规格:1ML 目录价:￥4132.44 市场价格:￥4132.44 会员价格:￥4132.44 到货时间:多数为现货, 2-3个工作日 数量: 购买发起团购 结束时间: ...
EZviewTM Red Protein A and ANTI-FLAG® M2 Affinity Gels: Immunoprecipitation with Enhanced Visibility Affinity BeadsThe strength and specificity of interactions between biomolecules have been exploited for years in biochemical research. Affinity-based protein purification techniques have been used ...
Affinity Pull-Down of Proteins Using Anti-FLAG M2 Agarose Beads. E Gerace,D Moazed - 《Methods in Enzymology》 被引量: 0发表: 2015年 Formation of a Carboxy-Terminal Domain Phosphatase (Fcp1)/TFIIF/RNA Polymerase II (pol II) Complex in Schizosaccharomyces pombe Involves Direct Interaction......
The pulled-down protein is then eluted from the beads by competition with a 3× FLAG peptide. Anti-FLAG antibodies specifically recognize the target protein within lysates with relatively low cross-reactivity with other cellular proteins; however, the M2 antibody can react with native protein ...
As such, there is no need to discuss the coupling of an anti-DYKDDDDK antibody to protein A or G beads. In addition, the DYKDDDDK Fab-Trap™ saves you experimental and hands-on time.For more information about the differences in IP using a Fab-fragment or an antibody, please see our ...
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Lina Gan, Zhichong Wu, Meilin Xue, Youwei Zhu, Cheng Xiong, Ting Wang, Xiaozhu Lin,et al.supernatant was collected forimmunoprecipitation. The supernatant was incubated with FLAG antibody (14793,Cell Signaling Technology),ALDOAantibody (11217-1-AP,...
Transfer 100 µL of M2 FLAG Affinity beads to the column and wash the gel away with 2 mL of FLAG-Purification Suspension Buffer. Dry the nose (bottom) of the column and close it with the cap very tightly, then transfer the supernatants from the centrifuged cell lysate to the column gen...