Intracellular fixation buffer for use in flow cytomerty, particularly for fixation in intracellular staining procedures. Composition is paraformaldehyde in phosphate深入閱讀 Have Questions? 联系我们產品號碼 FB001 價格(TWD)/ Each *** - 新增至購物車 Delivery information Standard Products - 1-2 busin...
Flow Cytometry Fixation Buffer (1X) Summary Specifications Source N/A Shipping Conditions The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. Storage Store the unopened product at 2 - 8 °C. Do not use past expiration date. ...
4. Add either 100 µl (for microwell plates) or 250 µl (for tubes) aliquots of fixation buffer to each cell pellet and resuspend the cells by either pipetting or vortexing. Incubate the cells with fixation buffer for 15 to 30 min at 4°C. (Cell aggregation can be avoided by vorte...
subsequent analysis by flow cytometry (or fluorescence microscopy). Note: BD Cytofix/Cytoperm™ solution (Cat. No. 554722) and the BD Perm/Wash™ buffer (Cat. No. 554723) are included in BD Cytofix/Cytoperm Kit (Cat. No. 554714) as well as the BD Cytofix/Cytoperm Plus Kit with ...
Intracellular Fixation and Permeabilization Buffer Set; eBioscience: Designed for use in intracellular staining and flow cytometric analysis and has been specially formulated to reduce non-specific staining of fluorochrome-labelled antibodies and increas
Since Hepes is not as noxious as cacodylate, we selected Hepes as the buffer for the fixative. Cell counting revealed that the decrease in cell numbers by fixation was negligible and did not start until at least 8 days after fixation. We successfully analysed the DNA amount in fixed cells ...
immunofluorescent staining in order to preserve the light-scattering signals and fluorescent intensities of cells for analysis at a later time. Cell Fixation Buffer may be useful to avoid the capping or shedding of fluorescent antibodies and/or surface antigens during the period before flow ...
Fixation Buffer 价格: ￥700.00 ￥700.0 评论:0条 收藏:0 产品详情 产品评论(0) 销售记录(0) 品牌:Biolegend型号:420801 规格:100 ml
Review how antibody clones for Flow Cytometry behave before and after fixation and permeabilization to help ensure you choose the best antibody clone for your experiment
SSC is a buffer commonly used in hybridization solution. It is reported that 5× SSC could be used as sheath fluid without dis- turbing cell-cycle flow analysis [18]. It does not affect the staining and sorting of fixed cells which have been stained with the specific DNA fluorochrome ...