Samples were incubated with 5 mg mL1 Phalloidin-FITC (green, Sigma-Aldrich) and 5 mg mL1 Hoechst 33258 (blue, Sigma-Aldrich) in culturemediumfor 20minutes at 37 C, then fixed with 3.7%paraformaldehyde for 30minutes and imaged under a Leica TCS-SP2 Confocal Microscope using TCS Leica ...