Fluorescein isothiocyanate (FITC) is widely used to attach a fluorescent label to proteins vi a the amine group. The isothiocyanate group reacts with amino terminal and primary amines in proteins. It has been used for the labeling of proteins including antibodies and lectins.2-6 Isomer I has ...
因此TUNEL (TdT mediated dUTP Nick End Labeling)细胞凋亡检测试剂盒可以用来检测组织细胞在凋亡晚期过程中细胞核DNA的断裂情况。其原理是在TdT酶的作用下,在基因组DNA断裂时暴露出的3´-OH末端掺入荧光素标记的dUTP(FITC-12-dUTP),从而可以用荧光显微镜或流式细胞仪检测(FITC激发450-500 nm,发射515-565 nm)...
TUNEL (TdT mediated dUTP Nick End Labeling)细胞凋亡检测试剂盒(FITC)可以用来检测组织细胞在凋亡晚期过程中细胞核DNA的断裂情况。其原理是在末端脱氧核糖核苷酸转移酶(Terminal Deoxynucleotidyl Transferase, TdT)的作用下,在基因组DNA断裂时暴露出的3′-羟基(3′-OH)末端掺入FITC-12-dUTP,从而可以用荧光显微镜或流...
包装规格:2 Labelings 品牌:AAT Bioquest 产地:American 硫氰酸相关产品报价 产品标题价格产地公司名称报价日期 硫氰酸胍,异硫氰酸电议湖北鸿鑫瑞宇精细化工有限公司2024-09-20询价 异硫氰酸胍/硫氰酸胍/胍硫氰酸盐/硫氰酸亚脲/...¥15/瓶南京VIP1年南京都莱生物技术有限公司2024-10-12询价 ...
proprietary buffer, pH 7.4-7.8, with 0.5% BSA, 30% glycerol 内含物 0.02% sodium azide 保存条件 -20° C, store in dark 运输条件 Ambient (domestic); Wet ice (international) 靶标信息 LCK is a member of the Src-family tyrosine kinase, which are essential for signaling through ...
PBS, pH 7.2, with 0.2% BSA 内含物 0.09% sodium azide 保存条件 4° C, store in dark, DO NOT FREEZE! 运输条件 Ambient (domestic); Wet ice (international) RRID AB_2572534 产品详细信息 Description: This CR3M monoclonal antibody reacts with human CCL3. CCL3, also known as MIP-1 alpha ...
BrdU labeling and staining with anti-BrdU antibody:1. Label dividing cells with 10 µM BrdU for 45 min at 37°C.2. Following the incubation, harvest the cells and wash once with 1X PBS.3. Stain surface molecules according to the Surface Staining Protocol.4. Wash in cold Flow Cytometry...
The use of optimal labeling conditions is recommended. Overlabeling of proteins generally results in altered specificity, aggregation and/or precipitation of the protein. Fluorescent labeling of antibodies with high fluorophore to antibody ratios (molar F/P >6) usually results in increased non-...
3046 rrid ab_2883828 conjugate fitc fluorescent dye excitation/emission maxima wavelengths 494 nm / 520 nm form liquid purification method protein g purification storage buffer pbs with 50% glycerol, 0.05% proclin300, 0.5% bsa, ph 7.3. storage conditions store at -20°c. avoid exposure to ...
Tip: If you need to save time, suspension cells can be attached to poly-D-lysine microplates or coverslips and then stained using the protocol for adherent cells. 3. Stain cultured cells with phalloidin conjugates Tip: Pre-incubating fixed cells with 1% BSA in PBS for 20–30 minutes may ...