Fluorescein isothiocyanate (FITC) is widely used to attach a fluorescent label to proteins vi a the amine group. The isothiocyanate group reacts with amino terminal and primary amines in proteins. It has been used for the labeling of proteins including antibodies and lectins.2-6 Isomer I has ...
FITC标记蛋白质的方法_生物学_自然科学_专业资料。? 1. FITC Labeling of Proteins ? The following protocol can be used to label proteins with concentr?... FITC标记蛋白 FITC标记蛋白_专业资料。FITC 标记蛋白 基本原理:蛋白质分子的赖氨酸残基的游离 ε-氨基可与 FITC 发生亲核反应,形成硫脲连接而共价结合...
Imaging protocol handbook Protocols that fit your needs in imaging ranging from sample and assay preparation to staining, labeling, and data analysis strategies. Request the handbook Fluorophore and reagent selection guide for cell imaging A tool for selecting the optimal fluorescent dyes for your Invit...
Thus, the Maxpar® labeling protocol would not work with Annexin V, unless a free –SH group can be introduced to Annexin V. For more information regarding SH-mediated conjugation of Annexin V please consult published papers such as this one. Certificate of Analysis Lot #: Customers Also...
PBS with 1% BSA 内含物 0.09% sodium azide 保存条件 Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Store in the dark. 运输条件 Ambient (domestic); Wet ice (international) RRID AB_2745282 产品详细信息 Mouse anti Bovine CD21...
Neither BSA nor amine buffers, in any chosen concentration, interfere with the labeling. 50% glycerol as well as preservatives like sodium azide are also compatible with the kit. Q: How many different primary antibodies can I label with one kit? A: You can label up to 50 different ...
包装规格:2 Labelings 货号:AAT-B1299 是否进口:是 ReadiLink FITC抗体标记检测试剂盒上海齐源生物科技有限公司 产品详细介绍:该试剂盒仅需要两个简单的混合步骤而无需纯化步骤。试剂盒中使用的iFluor和mFluor染料的琥珀酰亚胺酯(SE)显示出与蛋白质的脂族胺的良好反应性和选择性,并形成羧酰胺键,其与天然肽键相同...
PBS, pH 7.2, with 0.2% BSA 内含物 0.09% sodium azide 保存条件 4° C, store in dark, DO NOT FREEZE! 运输条件 Ambient (domestic); Wet ice (international) RRID AB_10597901 产品详细信息 Description: This SK11 monoclonal antibody reacts with human and non-human primate CD62L (also known ...
FITC-SiO2-COOH NPs function as a cell labeling agent in 2D culture Based on the in vitro angiogenesis assay results, the potential of FITC-SiO2-COOH NPs was assessed for their efficacy as a fluorescence labeling agent in live cell tracking. Two concentrations of the NPs were examined. Notably...
Label your antibody with fluorescein (FITC) just 15 minutes without a purification step. Labeling tolerates many common buffer components including BSA and ascites.