Ethanol-precipitate the TOPO® Cloning reaction and resuspend in water prior to electroporation Analysis of Positive Clones Pick 10 colonies and culture them overnight in LB medium containing 50 µg/ml ampicillin (3-5 ml). Isolate ...
Ethanol-precipitate the TOPO® Cloning reaction and resuspend in water prior to electroporation Analysis of Positive Clones Pick 10 colonies and culture them overnight in LB medium containing 50 µg/ml ampicillin (3-5 ml). Isolate ...
Remove inhibitors by ethanol precipitation of mRNA preparation before the firststrand reaction. Include a 70% (v/v) ethanol wash of the mRNA pellet. Note:Inhibitors of RT include sodium dodecyl sulfate (SDS), EDTA, guanidinium sa...
Ethanol-precipitate the TOPO® Cloning reaction and resuspend in water prior to electroporation Analysis of Positive Clones Pick 10 colonies and culture them overnight in LB medium containing 50 µg/ml ampicillin (3-5 ml). Isolate ...
Remove inhibitors by ethanol precipitation of mRNA preparation before the firststrand reaction. Include a 70% (v/v) ethanol wash of the mRNA pellet. Note:Inhibitors of RT include sodium dodecyl sulfate (SDS), EDTA, guanidinium sa...
Remove inhibitors by ethanol precipitation of mRNA preparation before the firststrand reaction. Include a 70% (v/v) ethanol wash of the mRNA pellet. Note:Inhibitors of RT include sodium dodecyl sulfate (SDS), EDTA, guanidinium sa...
Remove inhibitors by ethanol precipitation of mRNA preparation before the firststrand reaction. Include a 70% (v/v) ethanol wash of the mRNA pellet. Note:Inhibitors of RT include sodium dodecyl sulfate (SDS), EDTA, guanidinium sa...
Remove inhibitors by ethanol precipitation of mRNA preparation before the firststrand reaction. Include a 70% (v/v) ethanol wash of the mRNA pellet. Note: Inhibitors of RT include sodium dodecyl sulfate (SDS), EDTA, guanidinium...