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Absorbance was measured at 450 nm using a SpectraMax i3x plate reader (Molecular Devices, Sunnyvale, CA, USA). The toxins were three-fold diluted in the sample buffer, giving 10 standards from 0.007–131 ng mL-1 (AZA-1) and 0.0050–100 ng mL-1 (AZA-59), respectively. Two technical ...