To confirm the identity of the isolate, partial sequences of the internal transcribed spacer of rDNA region, glyceraldehyde-3-phosphate dehydrogenase, calmodulin, chitin synthase and actin genes were amplified (Weir et al. 2012) and sequenced (GenBank accession Nos. MH151141, MH151150, MH151136...
The isolate FDY-5 was chosen for molecular identification. The sequence of rDNA-ITS, TUB, and LSU of the FDY-5 were amplified (GenBank MT800929, MT799852, and MT800935, respectively) (White et al. 1990; Carbone and Kohn 1999; Lumbsch et al. 2000). BLAST results showed that the ...
Moreover, we also checked the 45S rDNA (ribosomal DNA) completeness in the assembly, as other species whose genome were built from second-generation sequencing; we also could not detect the complete 45S rDNA in the assembly32. This mostly came from the difficulty to assemble tandem repeat ...
Molecular phylogenetic analysis using SSU rDNA sequences from termite gregarine (black marked) and other eugregarines from various insects. The order of insect hosts was listed on the right of the tree. The tree was constructed by Maximum Likelihood method, and evolutionary analyses were conducted ...
Phylogenetic tree based on partial 16S rDNA sequences (1,247 bp) from the Convolvulus virgatus witches'-broom phytoplasma identified in this study (red rectangle) and selected phytoplasma sequences from GenBank (accession numbers in parentheses). Tree constructed by the neighbour-joining method using...
Evolutionary relationships between 15 Plasmodium species from New and Old World primates (including humans): a 18S rDNA cladistic analysis. Parasitol. 2004;129:677–84. Article CAS Google Scholar Ollomo B, Durand P, Prugnolle F, Douzery E, Arnathau C, Nkoghe D, et al. A new malaria...
(ReceivedJanuary25,2011.AcceptedFebruary18,2011) GraymoldofblueberrycausedbyBotrytissp.isreportedforthefirsttimeinKorea.Adetaileddescriptionofthefungus isgiven,alongwithitsrDNAinternaltranscribedspacersequence.ThefunguswasidentifiedasBotrytiscinereabasedon mycologicalcharacteristicsandmoleculardata. KEYWORDS:Botrytis...
The fungus was identified as Podosphaera tridactyla based on its anamorph and teleomorph characteristics (1,2). To confirm the identification, 28S rDNA and the ITS region were amplified. The ITS5/P3 and then PM5/ITS4 primers were used to amplify the ITS region by nested PCR. The ...
In this paper, 5S rDNA was mapped for the first time in Coleoptera using single and double FISH with 5S and 18S rDNAs as probes. Our results showed a single 5S rDNA site located in a different chromosome than the 18S rDNA sites. The presence of only one 5S rDNA site is common among ...
(2008). Evaluation of the bacterial diversity in the feces of cattle using 16S rDNA bacterial tag-encoded FLX amplicon pyrosequencing (bTEFAP). BMC Microbiol 8: 125. Egerton JR, Roberts DS, Parsonson IM. (1969). The aetiology and pathogenesis of ovine foot-rot. I. A histological study of...